Abstract
A selective trans-packaging system was developed to produce and isolate bovine viral diarrhea virus (BVDV) pseudo-particles with complementing reporter replicons and their packaging proteins expressed in trans with recombinant vaccinia virus. The encapsidation of replicon rNS3-5B was dependent not only on the in trans expression of structural proteins C, Erns, E1 and E2, but also the nonstructural proteins, p7 and contiguous precursor NS2-3-4A. Nonstructural p7, NS4B, NS5A or NS5B could be expressed in cis and in trans with precursor NS2-3-4A without significantly affecting virion assembly efficiency. NS2-3-4A was identified as an in trans functional precursor in virion assembly. BVDV genomes with mutant NS5B, which did not undergo active replication, were packaged 5-fold less efficiently than the intact genomes demonstrating the importance of replication in virion packaging. These results suggest that genome replication and assembly are closely associated, consistent with a model in which these two steps are coupled for maximum efficiency.
Original language | English (US) |
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Pages (from-to) | 331-340 |
Number of pages | 10 |
Journal | Virology |
Volume | 387 |
Issue number | 2 |
DOIs | |
State | Published - May 10 2009 |
Keywords
- BVDV
- Complementation
- NS2-3-4A precursor
- Pseudo-particles
- Replication
- Trans-packaging
- Virion assembly
ASJC Scopus subject areas
- Virology