TY - JOUR
T1 - A reversed phase ultra-high-performance liquid chromatography-data independent mass spectrometry method for the rapid identification of mycobacterial lipids
AU - Sakallioglu, Isin T.
AU - Maroli, Amith S.
AU - Leite, Aline De Lima
AU - Powers, Robert
N1 - Funding Information:
This material is based upon work supported by the National Science Foundation under Grant Number 1660921 and was supported in part by funding from the Nebraska Center for Integrated Biomolecular Communication (P20GM113126, NIGMS). The research was performed in facilities renovated with support from the National Institutes of Health (RR015468-01).
Funding Information:
This material is based upon work supported by the National Science Foundation under Grant Number 1660921 and was supported in part by funding from the Nebraska Center for Integrated Biomolecular Communication ( P20GM113126 , NIGMS). The research was performed in facilities renovated with support from the National Institutes of Health ( RR015468-01 ).
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2022/1/11
Y1 - 2022/1/11
N2 - A rapid reversed-phase ultra-high-performance liquid chromatography-high resolution mass spectrometry based mycobacterial lipidomics approach is described. This method enables the separation of various lipid classes including lipids specific to mycobacterial, such as methoxy mycolic acid and α-mycolic acid. Lipid separation occurs during a relatively short runtime of 14 min on a charged surface hybrid C18 column. A high-resolution quadrupole-time of flight mass spectrometer and a data independent acquisition mode allowed for the simultaneous acquisition of the full scan and collision induced dissociation fragmentation. The proposed method provides lipid detection results equivalent to or better than existing methods, but with a faster throughput and an overall higher sensitivity. The reversed-phase ultra-high-performance liquid chromatography-high resolution mass spectrometry method was shown to obtain structural information for lipids extracted from Mycobacterium smegmatis, but the method is applicable to the analysis of lipids from various bacterial and mammalian cell lines.
AB - A rapid reversed-phase ultra-high-performance liquid chromatography-high resolution mass spectrometry based mycobacterial lipidomics approach is described. This method enables the separation of various lipid classes including lipids specific to mycobacterial, such as methoxy mycolic acid and α-mycolic acid. Lipid separation occurs during a relatively short runtime of 14 min on a charged surface hybrid C18 column. A high-resolution quadrupole-time of flight mass spectrometer and a data independent acquisition mode allowed for the simultaneous acquisition of the full scan and collision induced dissociation fragmentation. The proposed method provides lipid detection results equivalent to or better than existing methods, but with a faster throughput and an overall higher sensitivity. The reversed-phase ultra-high-performance liquid chromatography-high resolution mass spectrometry method was shown to obtain structural information for lipids extracted from Mycobacterium smegmatis, but the method is applicable to the analysis of lipids from various bacterial and mammalian cell lines.
KW - Lipidomics
KW - Liquid chromatography
KW - Mass spectrometry
KW - Mycobacteria
KW - Mycolic acid
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U2 - 10.1016/j.chroma.2021.462739
DO - 10.1016/j.chroma.2021.462739
M3 - Article
C2 - 34929571
AN - SCOPUS:85121238429
SN - 0021-9673
VL - 1662
JO - Journal of Chromatography A
JF - Journal of Chromatography A
M1 - 462739
ER -