A role for G-CSF receptor signaling in the regulation of hematopoietic cell function but not lineage commitment or differentiation

Craig L. Semerad, Jennifer Poursine-Laurent, Fulu Liu, Daniel C. Link

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

To investigate the specificity of cytokine signals in hematopoietic differentiation, we generated mice with a targeted mutation of their G-CSF receptor (G-CSFR) such that the cytoplasmic (signaling) domain of the G-CSFR is replaced with the cytoplasmic domain of the erythropoietin receptor. In homozygous mutant mice, expression of this chimeric receptor had no apparent affect on lineage commitment and was able to support the production of morphologically mature neutrophils. However, mutant neutrophils displayed reduced chemotaxis, and G-CSF-stimulated mobilization of neutrophils and hematopoietic progenitors from the bone marrow to blood was markedly impaired. Thus, the G-CSFR is generating unique signals that are required for certain specialized hematopoietic cell functions but are not required for granulocytic differentiation or lineage commitment.

Original languageEnglish (US)
Pages (from-to)153-161
Number of pages9
JournalImmunity
Volume11
Issue number2
DOIs
StatePublished - Aug 1999

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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