A role for she, Grb2, and Raf-1 in FcγRI signal relay

Rae Kil Park, Yenbou Liu, Donald L. Durden

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

The activation of the serine/threonine kinase, Raf-1, serves to connect upstream protein tyrosine kinases to downstream signaling events. We previously reported that FcγRI stimulation of Interferon γ-differentiated U937 cells (termed U937IF cells) induces a mobility shift in Erk2. Herein, we report that cross-linking of FcγRI receptor in U937IF cells induces a marked tyrosine phosphorylation of Raf-1 (10-fold increase). Tyrosine phosphorylation of Raf-1 is induced by FcγRI activation and not by PMA (1 μg/ml), N-formyl-Met-Leu-Phe (1 μM), calcium ionophore (1 μM), thrombin (0.05 unit/ml), FcγRII, or FcγRIII stimulation. The kinetics of Raf-1 tyrosine phosphorylation is rapid, reaching peak levels 1-2 min after FcγRI activation, and the tyrosine phosphorylation of Raf-1 precedes the activation of the respiratory burst. FcγRI cross-linking induces the tyrosine phosphorylation of Shc; tyrosine-phosphorylated She binds to Grb2 forming a Shc-Grb2 complex. The data provide evidence that the FcγRI receptor signals via the upstream activation of nonreceptor protein tyrosine kinases, which leads to the subsequent activation of Ras family GTPases and serine/threonine kinases, Raf-1 and mitogen-activated protein kinase.

Original languageEnglish (US)
Pages (from-to)13342-13348
Number of pages7
JournalJournal of Biological Chemistry
Volume271
Issue number23
DOIs
StatePublished - 1996

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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