TY - JOUR
T1 - A Scalable and Efficient Bioprocess for Manufacturing Human Pluripotent Stem Cell-Derived Endothelial Cells
AU - Lin, Haishuang
AU - Du, Qian
AU - Li, Qiang
AU - Wang, Ou
AU - Wang, Zhanqi
AU - Sahu, Neety
AU - Elowsky, Christian
AU - Liu, Kan
AU - Zhang, Chi
AU - Chung, Soonkyu
AU - Duan, Bin
AU - Lei, Yuguo
N1 - Publisher Copyright:
© 2018 The Authors
PY - 2018/8/14
Y1 - 2018/8/14
N2 - Endothelial cells (ECs) are of great value for cell therapy, tissue engineering, and drug discovery. Obtaining high-quantity and -quality ECs remains very challenging. Here, we report a method for the scalable manufacturing of ECs from human pluripotent stem cells (hPSCs). hPSCs are expanded and differentiated into ECs in a 3D thermoreversible PNIPAAm-PEG hydrogel. The hydrogel protects cells from hydrodynamic stresses in the culture vessel and prevents cells from excessive agglomeration, leading to high-culture efficiency including high-viability (>90%), high-purity (>80%), and high-volumetric yield (2.0 × 107 cells/mL). These ECs (i.e., 3D-ECs) had similar properties as ECs made using 2D culture systems (i.e., 2D-ECs). Genome-wide gene expression analysis showed that 3D-ECs had higher expression of genes related to vasculature development, extracellular matrix, and glycolysis, while 2D-ECs had higher expression of genes related to cell proliferation. Endothelial cells (ECs) differentiated from human pluripotent stem cells (hPSCs) are needed in large numbers for regenerative medicine. In this article, Lei and colleagues describe a simple and innovative method for the scalable production of ECs from hPSCs. The method has potential to make ECs broadly available and affordable for various applications.
AB - Endothelial cells (ECs) are of great value for cell therapy, tissue engineering, and drug discovery. Obtaining high-quantity and -quality ECs remains very challenging. Here, we report a method for the scalable manufacturing of ECs from human pluripotent stem cells (hPSCs). hPSCs are expanded and differentiated into ECs in a 3D thermoreversible PNIPAAm-PEG hydrogel. The hydrogel protects cells from hydrodynamic stresses in the culture vessel and prevents cells from excessive agglomeration, leading to high-culture efficiency including high-viability (>90%), high-purity (>80%), and high-volumetric yield (2.0 × 107 cells/mL). These ECs (i.e., 3D-ECs) had similar properties as ECs made using 2D culture systems (i.e., 2D-ECs). Genome-wide gene expression analysis showed that 3D-ECs had higher expression of genes related to vasculature development, extracellular matrix, and glycolysis, while 2D-ECs had higher expression of genes related to cell proliferation. Endothelial cells (ECs) differentiated from human pluripotent stem cells (hPSCs) are needed in large numbers for regenerative medicine. In this article, Lei and colleagues describe a simple and innovative method for the scalable production of ECs from hPSCs. The method has potential to make ECs broadly available and affordable for various applications.
KW - 3D culture
KW - endothelial cells
KW - human pluripotent stem cells
KW - thermoreversible hydrogel
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U2 - 10.1016/j.stemcr.2018.07.001
DO - 10.1016/j.stemcr.2018.07.001
M3 - Article
C2 - 30078557
AN - SCOPUS:85053845670
SN - 2213-6711
VL - 11
SP - 454
EP - 469
JO - Stem Cell Reports
JF - Stem Cell Reports
IS - 2
ER -