Bio cell manipulation is becoming an important area of interest. The current manipulation activities are mostly empirically driven approaches. There is a need to develop an integrated cell manipulation framework. Meanwhile, as the development of information technology (IT) and World Wide Web (WWW), it would be useful to create a 'semantic' web which would enable the computers explicitly and software tools implicitly to understand the requests provided by users. The paper presents a semantic web based integrated framework for bio cell manipulation. The design of the overall approach is described using engineering Enterprise Modeling Language (eEML). With the identified life cycle for bio cell manipulation, a 3-world model for bio cell manipulation (3WMBM) is delineated with the necessary hardware units and software modules.