A sensitive and selective LC-MS/MS method for quantitation of ivermectin in human, mouse and monkey plasma: Clinical validation

Yashpal S. Chhonker, Liping Ma, Constant Edi, Daryl J. Murry

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: A sensitive and selective LC-MS/MS method was validated for quantitation of ivermectin (IVM) in plasma. Method: The IVM was extracted from plasma using solid-phase extraction with C-18 cartridges. Separation of analytes was achieved on an ACE C18 column with isocratic elution using 0.1% acetic acid and methanol: acetonitrile (1:1, v/v) as mobile phase. The IVM was quantitated using electrospray ionization operating in negative multiple reaction monitoring mode. Results: The MS/MS response was linear over the concentration range from 0.1-1000 ng/ml. The method for human plasma was validated as per US FDA guidelines. The LC-MS/MS method is sensitive, reproducible, has easy sample preparation and is suitable for IVM quantitation in clinical samples.

Original languageEnglish (US)
Pages (from-to)1841-1852
Number of pages12
JournalBioanalysis
Volume10
Issue number22
DOIs
StatePublished - 2018

Keywords

  • Abamectin
  • Ivermectin
  • LC-MS/MS
  • Pharmacokinetics

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Clinical Biochemistry
  • Medical Laboratory Technology

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