A sensitive and selective LC-MS/MS method for quantitation of ivermectin in human, mouse and monkey plasma: Clinical validation

Yashpal S. Chhonker; Liping Ma; Constant Edi; Daryl J. Murry

doi:10.4155/bio-2018-0110

A sensitive and selective LC-MS/MS method for quantitation of ivermectin in human, mouse and monkey plasma: Clinical validation

Yashpal S. Chhonker, Liping Ma, Constant Edi, Daryl J. Murry

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Aim: A sensitive and selective LC-MS/MS method was validated for quantitation of ivermectin (IVM) in plasma. Method: The IVM was extracted from plasma using solid-phase extraction with C-18 cartridges. Separation of analytes was achieved on an ACE C18 column with isocratic elution using 0.1% acetic acid and methanol: acetonitrile (1:1, v/v) as mobile phase. The IVM was quantitated using electrospray ionization operating in negative multiple reaction monitoring mode. Results: The MS/MS response was linear over the concentration range from 0.1-1000 ng/ml. The method for human plasma was validated as per US FDA guidelines. The LC-MS/MS method is sensitive, reproducible, has easy sample preparation and is suitable for IVM quantitation in clinical samples.

Original language	English (US)
Pages (from-to)	1841-1852
Number of pages	12
Journal	Bioanalysis
Volume	10
Issue number	22
DOIs	https://doi.org/10.4155/bio-2018-0110
State	Published - 2018

Keywords

Abamectin
Ivermectin
LC-MS/MS
Pharmacokinetics

ASJC Scopus subject areas

Analytical Chemistry
General Pharmacology, Toxicology and Pharmaceutics
Medical Laboratory Technology
Clinical Biochemistry

Access to Document

10.4155/bio-2018-0110

Cite this

@article{81552abf69d841c993d2e324cbe5dc68,

title = "A sensitive and selective LC-MS/MS method for quantitation of ivermectin in human, mouse and monkey plasma: Clinical validation",

abstract = "Aim: A sensitive and selective LC-MS/MS method was validated for quantitation of ivermectin (IVM) in plasma. Method: The IVM was extracted from plasma using solid-phase extraction with C-18 cartridges. Separation of analytes was achieved on an ACE C18 column with isocratic elution using 0.1% acetic acid and methanol: acetonitrile (1:1, v/v) as mobile phase. The IVM was quantitated using electrospray ionization operating in negative multiple reaction monitoring mode. Results: The MS/MS response was linear over the concentration range from 0.1-1000 ng/ml. The method for human plasma was validated as per US FDA guidelines. The LC-MS/MS method is sensitive, reproducible, has easy sample preparation and is suitable for IVM quantitation in clinical samples.",

keywords = "Abamectin, Ivermectin, LC-MS/MS, Pharmacokinetics",

author = "Chhonker, {Yashpal S.} and Liping Ma and Constant Edi and Murry, {Daryl J.}",

note = "Publisher Copyright: {\textcopyright} 2018 Newlands Press.",

year = "2018",

doi = "10.4155/bio-2018-0110",

language = "English (US)",

volume = "10",

pages = "1841--1852",

journal = "Bioanalysis",

issn = "1757-6180",

publisher = "Taylor and Francis Ltd.",

number = "22",

}

TY - JOUR

T1 - A sensitive and selective LC-MS/MS method for quantitation of ivermectin in human, mouse and monkey plasma

T2 - Clinical validation

AU - Chhonker, Yashpal S.

AU - Ma, Liping

AU - Edi, Constant

AU - Murry, Daryl J.

PY - 2018

Y1 - 2018

N2 - Aim: A sensitive and selective LC-MS/MS method was validated for quantitation of ivermectin (IVM) in plasma. Method: The IVM was extracted from plasma using solid-phase extraction with C-18 cartridges. Separation of analytes was achieved on an ACE C18 column with isocratic elution using 0.1% acetic acid and methanol: acetonitrile (1:1, v/v) as mobile phase. The IVM was quantitated using electrospray ionization operating in negative multiple reaction monitoring mode. Results: The MS/MS response was linear over the concentration range from 0.1-1000 ng/ml. The method for human plasma was validated as per US FDA guidelines. The LC-MS/MS method is sensitive, reproducible, has easy sample preparation and is suitable for IVM quantitation in clinical samples.

AB - Aim: A sensitive and selective LC-MS/MS method was validated for quantitation of ivermectin (IVM) in plasma. Method: The IVM was extracted from plasma using solid-phase extraction with C-18 cartridges. Separation of analytes was achieved on an ACE C18 column with isocratic elution using 0.1% acetic acid and methanol: acetonitrile (1:1, v/v) as mobile phase. The IVM was quantitated using electrospray ionization operating in negative multiple reaction monitoring mode. Results: The MS/MS response was linear over the concentration range from 0.1-1000 ng/ml. The method for human plasma was validated as per US FDA guidelines. The LC-MS/MS method is sensitive, reproducible, has easy sample preparation and is suitable for IVM quantitation in clinical samples.

KW - Abamectin

KW - Ivermectin

KW - LC-MS/MS

KW - Pharmacokinetics

UR - http://www.scopus.com/inward/record.url?scp=85057749590&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85057749590&partnerID=8YFLogxK

U2 - 10.4155/bio-2018-0110

DO - 10.4155/bio-2018-0110

M3 - Article

C2 - 30325218

AN - SCOPUS:85057749590

SN - 1757-6180

VL - 10

SP - 1841

EP - 1852

JO - Bioanalysis

JF - Bioanalysis

IS - 22

ER -

A sensitive and selective LC-MS/MS method for quantitation of ivermectin in human, mouse and monkey plasma: Clinical validation

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this