A short N-terminal sequence of PTEN controls cytoplasmic localization and is required for suppression of cell growth

G. Denning, B. Jean-Joseph, C. Prince, D. L. Durden, P. K. Vogt

Research output: Contribution to journalArticlepeer-review

80 Scopus citations

Abstract

Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is an important negative regulator of cell growth and a tumor suppressor. Its growth-attenuating activity is based on the dephosphorylation of phosphatidylinositol 3,4,5-trisphosphate (PIP3), an essential second messenger for the phosphoinositide 3-kinase/Akt signaling pathway. This activity may require localization of PTEN to cytoplasmic membranes. Yet PTEN can also localize to the cell nucleus where its functions remain unclear. Here we present data that define a short sequence in the N-terminal region of PTEN required for cytoplasmic localization. We will refer to this sequence as cytoplasmic localization signal (CLS). It could function as a non-canonical signal for nuclear export or as a cytoplasmic retention signal of PTEN. Mutations within the CLS induce nuclear localization and impair growth suppressive activities of PTEN while preserving lipid phosphatase activity. We propose that nuclear localization of PTEN is not compatible with plasma membrane-targeted growth suppressive functions of PTEN.

Original languageEnglish (US)
Pages (from-to)3930-3940
Number of pages11
JournalOncogene
Volume26
Issue number27
DOIs
StatePublished - Jun 7 2007

Keywords

  • Cytoplasmic localization signal
  • Lipid binding
  • Mutation
  • PTEN
  • Tumor suppressor

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Fingerprint Dive into the research topics of 'A short N-terminal sequence of PTEN controls cytoplasmic localization and is required for suppression of cell growth'. Together they form a unique fingerprint.

Cite this