A simplified method for combined immunohistochemistry and in-situ hybridization in fresh-frozen, cryocut mouse brain sections

Keyphrases

• Immunohistochemistry 100%
• In Situ Hybridization 100%
• Simplified Method 100%
• Mouse Brain Section 100%
• Fresh-frozen 100%
• Gene Expression 50%
• Mutant Forms 50%
• CAMP Response Element-binding Protein 50%
• Transgenic 25%
• Ribonuclease 25%
• Phosphorylation 25%
• Microarray Analysis 25%
• Perfusion 25%
• Colocalization 25%
• Brain Regions 25%
• Paraffin Embedding 25%
• Double Transgenic Mice 25%
• Dominant-negative mutant 25%
• Paraformaldehyde 25%
• Post-translational Modification 25%
• Enkephalin 25%
• Real-time PCR Analysis 25%
• Downstream Targets 25%
• Dynorphin 25%
• Cell Layer 25%
• Background Signal 25%
• Glass Slide 25%
• Reliable Detection 25%
• Cryostat 25%
• Immunodetection 25%
• Background Interference 25%

Engineering

• In Situ Hybridization 100%

Neuroscience

• Cryostat 25%