TY - JOUR
T1 - A single amino acid change restores DNA cytosine methyltransferase activity in a cloned chlorella virus pseudogene
AU - Zhang, Yanping
AU - Nelson, Michael
AU - Van Etten, James L.
N1 - Funding Information:
We thank Les Lane and Roy French for helpful discussions, Dwight Burbank for technical assistance, and Ira Schildkraut for a gift of Agel restriction endonuclease. This investigation was supported, in part, by Public Health Service Grant GM-32441 from the National Institute of General Medical Sciences. This manuscript has been assigned Journal Series No. 9800, Agricultural Research Division, University of Nebraska.
PY - 1992/4/11
Y1 - 1992/4/11
N2 - The chlorelia virus PBCV-1 contains an open reading frame, named P17-ORF4, which differs by eight amino acids from a DNA cytosine methyltransferase, M.CviJI, encoded by a different chlorelia virus IL-3A. Whereas IL-3A expresses M.CviJI, which methylates the central cytosine in (A/G)GCT/C/G) sequences, P17-ORF4 is non-functional. Gene fusions between P17-ORF4 and M.CvUI and site-directed point mutations revealed that changing Gin188 to Lys188 abolishes M.CviJI methyltransferase activity. Conversely, changing Lys188 in P17-ORF4 to Gin188 results in M.CvUI activity. The other altered seven amino acids do not appear to affect M.CviJI activity.
AB - The chlorelia virus PBCV-1 contains an open reading frame, named P17-ORF4, which differs by eight amino acids from a DNA cytosine methyltransferase, M.CviJI, encoded by a different chlorelia virus IL-3A. Whereas IL-3A expresses M.CviJI, which methylates the central cytosine in (A/G)GCT/C/G) sequences, P17-ORF4 is non-functional. Gene fusions between P17-ORF4 and M.CvUI and site-directed point mutations revealed that changing Gin188 to Lys188 abolishes M.CviJI methyltransferase activity. Conversely, changing Lys188 in P17-ORF4 to Gin188 results in M.CvUI activity. The other altered seven amino acids do not appear to affect M.CviJI activity.
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U2 - 10.1093/nar/20.7.1637
DO - 10.1093/nar/20.7.1637
M3 - Article
C2 - 1579454
AN - SCOPUS:0026555466
VL - 20
SP - 1637
EP - 1642
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 7
ER -