A single amino acid change restores DNA cytosine methyltransferase activity in a cloned chlorella virus pseudogene

Yanping Zhang, Michael Nelson, James L. Van Etten

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The chlorelia virus PBCV-1 contains an open reading frame, named P17-ORF4, which differs by eight amino acids from a DNA cytosine methyltransferase, M.CviJI, encoded by a different chlorelia virus IL-3A. Whereas IL-3A expresses M.CviJI, which methylates the central cytosine in (A/G)GCT/C/G) sequences, P17-ORF4 is non-functional. Gene fusions between P17-ORF4 and M.CvUI and site-directed point mutations revealed that changing Gin188 to Lys188 abolishes M.CviJI methyltransferase activity. Conversely, changing Lys188 in P17-ORF4 to Gin188 results in M.CvUI activity. The other altered seven amino acids do not appear to affect M.CviJI activity.

Original languageEnglish (US)
Pages (from-to)1637-1642
Number of pages6
JournalNucleic acids research
Volume20
Issue number7
DOIs
StatePublished - Apr 11 1992

ASJC Scopus subject areas

  • Genetics

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