A single amino acid change restores DNA cytosine methyltransferase activity in a cloned chlorella virus pseudogene

Yanping Zhang; Michael Nelson; James L. Van Etten

doi:10.1093/nar/20.7.1637

A single amino acid change restores DNA cytosine methyltransferase activity in a cloned chlorella virus pseudogene

Yanping Zhang, Michael Nelson, James L. Van Etten

Plant Pathology

Research output: Contribution to journal › Article

16 Scopus citations

Abstract

The chlorelia virus PBCV-1 contains an open reading frame, named P17-ORF4, which differs by eight amino acids from a DNA cytosine methyltransferase, M.CviJI, encoded by a different chlorelia virus IL-3A. Whereas IL-3A expresses M.CviJI, which methylates the central cytosine in (A/G)GCT/C/G) sequences, P17-ORF4 is non-functional. Gene fusions between P17-ORF4 and M.CvUI and site-directed point mutations revealed that changing Gin¹⁸⁸ to Lys¹⁸⁸ abolishes M.CviJI methyltransferase activity. Conversely, changing Lys¹⁸⁸ in P17-ORF4 to Gin¹⁸⁸ results in M.CvUI activity. The other altered seven amino acids do not appear to affect M.CviJI activity.

Original language	English (US)
Pages (from-to)	1637-1642
Number of pages	6
Journal	Nucleic acids research
Volume	20
Issue number	7
DOIs	https://doi.org/10.1093/nar/20.7.1637
State	Published - Apr 11 1992

ASJC Scopus subject areas

Genetics

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Zhang, Y., Nelson, M., & Van Etten, J. L. (1992). A single amino acid change restores DNA cytosine methyltransferase activity in a cloned chlorella virus pseudogene. Nucleic acids research, 20(7), 1637-1642. https://doi.org/10.1093/nar/20.7.1637

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title = "A single amino acid change restores DNA cytosine methyltransferase activity in a cloned chlorella virus pseudogene",

abstract = "The chlorelia virus PBCV-1 contains an open reading frame, named P17-ORF4, which differs by eight amino acids from a DNA cytosine methyltransferase, M.CviJI, encoded by a different chlorelia virus IL-3A. Whereas IL-3A expresses M.CviJI, which methylates the central cytosine in (A/G)GCT/C/G) sequences, P17-ORF4 is non-functional. Gene fusions between P17-ORF4 and M.CvUI and site-directed point mutations revealed that changing Gin188 to Lys188 abolishes M.CviJI methyltransferase activity. Conversely, changing Lys188 in P17-ORF4 to Gin188 results in M.CvUI activity. The other altered seven amino acids do not appear to affect M.CviJI activity.",

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AB - The chlorelia virus PBCV-1 contains an open reading frame, named P17-ORF4, which differs by eight amino acids from a DNA cytosine methyltransferase, M.CviJI, encoded by a different chlorelia virus IL-3A. Whereas IL-3A expresses M.CviJI, which methylates the central cytosine in (A/G)GCT/C/G) sequences, P17-ORF4 is non-functional. Gene fusions between P17-ORF4 and M.CvUI and site-directed point mutations revealed that changing Gin188 to Lys188 abolishes M.CviJI methyltransferase activity. Conversely, changing Lys188 in P17-ORF4 to Gin188 results in M.CvUI activity. The other altered seven amino acids do not appear to affect M.CviJI activity.

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