A single amino acid change restores DNA cytosine methyltransferase activity in a cloned chlorella virus pseudogene

Yanping Zhang; Michael Nelson; James L. Van Etten

doi:10.1093/nar/20.7.1637

A single amino acid change restores DNA cytosine methyltransferase activity in a cloned chlorella virus pseudogene

Yanping Zhang, Michael Nelson, James L. Van Etten

Plant Pathology

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The chlorelia virus PBCV-1 contains an open reading frame, named P17-ORF4, which differs by eight amino acids from a DNA cytosine methyltransferase, M.CviJI, encoded by a different chlorelia virus IL-3A. Whereas IL-3A expresses M.CviJI, which methylates the central cytosine in (A/G)GCT/C/G) sequences, P17-ORF4 is non-functional. Gene fusions between P17-ORF4 and M.CvUI and site-directed point mutations revealed that changing Gin¹⁸⁸ to Lys¹⁸⁸ abolishes M.CviJI methyltransferase activity. Conversely, changing Lys¹⁸⁸ in P17-ORF4 to Gin¹⁸⁸ results in M.CvUI activity. The other altered seven amino acids do not appear to affect M.CviJI activity.

Original language	English (US)
Pages (from-to)	1637-1642
Number of pages	6
Journal	Nucleic acids research
Volume	20
Issue number	7
DOIs	https://doi.org/10.1093/nar/20.7.1637
State	Published - Apr 11 1992

ASJC Scopus subject areas

Genetics

Access to Document

10.1093/nar/20.7.1637

Fingerprint Dive into the research topics of 'A single amino acid change restores DNA cytosine methyltransferase activity in a cloned chlorella virus pseudogene'. Together they form a unique fingerprint.

Cite this

@article{0625378ae8ec44f7aa97d64cd1143d00,

title = "A single amino acid change restores DNA cytosine methyltransferase activity in a cloned chlorella virus pseudogene",

abstract = "The chlorelia virus PBCV-1 contains an open reading frame, named P17-ORF4, which differs by eight amino acids from a DNA cytosine methyltransferase, M.CviJI, encoded by a different chlorelia virus IL-3A. Whereas IL-3A expresses M.CviJI, which methylates the central cytosine in (A/G)GCT/C/G) sequences, P17-ORF4 is non-functional. Gene fusions between P17-ORF4 and M.CvUI and site-directed point mutations revealed that changing Gin188 to Lys188 abolishes M.CviJI methyltransferase activity. Conversely, changing Lys188 in P17-ORF4 to Gin188 results in M.CvUI activity. The other altered seven amino acids do not appear to affect M.CviJI activity.",

author = "Yanping Zhang and Michael Nelson and {Van Etten}, {James L.}",

note = "Funding Information: We thank Les Lane and Roy French for helpful discussions, Dwight Burbank for technical assistance, and Ira Schildkraut for a gift of Agel restriction endonuclease. This investigation was supported, in part, by Public Health Service Grant GM-32441 from the National Institute of General Medical Sciences. This manuscript has been assigned Journal Series No. 9800, Agricultural Research Division, University of Nebraska.",

year = "1992",

month = apr,

day = "11",

doi = "10.1093/nar/20.7.1637",

language = "English (US)",

volume = "20",

pages = "1637--1642",

journal = "Nucleic Acids Research",

issn = "0305-1048",

publisher = "Oxford University Press",

number = "7",

}

TY - JOUR

T1 - A single amino acid change restores DNA cytosine methyltransferase activity in a cloned chlorella virus pseudogene

AU - Zhang, Yanping

AU - Nelson, Michael

AU - Van Etten, James L.

N1 - Funding Information: We thank Les Lane and Roy French for helpful discussions, Dwight Burbank for technical assistance, and Ira Schildkraut for a gift of Agel restriction endonuclease. This investigation was supported, in part, by Public Health Service Grant GM-32441 from the National Institute of General Medical Sciences. This manuscript has been assigned Journal Series No. 9800, Agricultural Research Division, University of Nebraska.

PY - 1992/4/11

Y1 - 1992/4/11

N2 - The chlorelia virus PBCV-1 contains an open reading frame, named P17-ORF4, which differs by eight amino acids from a DNA cytosine methyltransferase, M.CviJI, encoded by a different chlorelia virus IL-3A. Whereas IL-3A expresses M.CviJI, which methylates the central cytosine in (A/G)GCT/C/G) sequences, P17-ORF4 is non-functional. Gene fusions between P17-ORF4 and M.CvUI and site-directed point mutations revealed that changing Gin188 to Lys188 abolishes M.CviJI methyltransferase activity. Conversely, changing Lys188 in P17-ORF4 to Gin188 results in M.CvUI activity. The other altered seven amino acids do not appear to affect M.CviJI activity.

AB - The chlorelia virus PBCV-1 contains an open reading frame, named P17-ORF4, which differs by eight amino acids from a DNA cytosine methyltransferase, M.CviJI, encoded by a different chlorelia virus IL-3A. Whereas IL-3A expresses M.CviJI, which methylates the central cytosine in (A/G)GCT/C/G) sequences, P17-ORF4 is non-functional. Gene fusions between P17-ORF4 and M.CvUI and site-directed point mutations revealed that changing Gin188 to Lys188 abolishes M.CviJI methyltransferase activity. Conversely, changing Lys188 in P17-ORF4 to Gin188 results in M.CvUI activity. The other altered seven amino acids do not appear to affect M.CviJI activity.

UR - http://www.scopus.com/inward/record.url?scp=0026555466&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026555466&partnerID=8YFLogxK

U2 - 10.1093/nar/20.7.1637

DO - 10.1093/nar/20.7.1637

M3 - Article

C2 - 1579454

AN - SCOPUS:0026555466

VL - 20

SP - 1637

EP - 1642

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 7

ER -