A site-specific single strand endonuclease activity induced by NYs-1 virus infection of a Chlorella-like green alga

Yuannan Xia; Richard Morgan; Ira Schildkraut; James L. van Etten

doi:10.1093/nar/16.20.9477

A site-specific single strand endonuclease activity induced by NYs-1 virus infection of a Chlorella-like green alga

Yuannan Xia, Richard Morgan, Ira Schildkraut, James L. van Etten

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

A site-specific endonuclease was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA-containing virus NYs-1. The enzyme recognizes the sequence 5'-CC-3' and cleaves 5' to the first C. It cleaves 5'-C^mC-3' sequences but not 5'-^mCC-3' sequences. The enzyme creates breaks in dsDNA whenever two 5'-CC-3' sequences on opposite strands are close enough for the two strands to separate; when the 5'-CC-3' sequences on opposite strands are further apart only a portion of the strands separate. Consequently, NYs-1 endonuclease does not produce a completely stable DNA digestion pattern. The enzyme probably does not cleave ssDNA and definitely does not cleave ssRNA or dsRNA.

Original language	English (US)
Pages (from-to)	9477-9487
Number of pages	11
Journal	Nucleic acids research
Volume	16
Issue number	20
DOIs	https://doi.org/10.1093/nar/16.20.9477
State	Published - Oct 25 1988

ASJC Scopus subject areas

Genetics

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title = "A site-specific single strand endonuclease activity induced by NYs-1 virus infection of a Chlorella-like green alga",

abstract = "A site-specific endonuclease was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA-containing virus NYs-1. The enzyme recognizes the sequence 5'-CC-3' and cleaves 5' to the first C. It cleaves 5'-CmC-3' sequences but not 5'-mCC-3' sequences. The enzyme creates breaks in dsDNA whenever two 5'-CC-3' sequences on opposite strands are close enough for the two strands to separate; when the 5'-CC-3' sequences on opposite strands are further apart only a portion of the strands separate. Consequently, NYs-1 endonuclease does not produce a completely stable DNA digestion pattern. The enzyme probably does not cleave ssDNA and definitely does not cleave ssRNA or dsRNA.",

author = "Yuannan Xia and Richard Morgan and Ira Schildkraut and {van Etten}, {James L.}",

note = "Funding Information: ACKNOWLEDGEMENTS We thank Rich Roberts andStuart Linn for helpful discussions. The investigation was supported, in part, by Public Health Service grant GM-32441 from the National Institute of General Medicine and grant DE-ACO2-82ER12086 from the Department of Energy.",

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doi = "10.1093/nar/16.20.9477",

language = "English (US)",

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T1 - A site-specific single strand endonuclease activity induced by NYs-1 virus infection of a Chlorella-like green alga

AU - Xia, Yuannan

AU - Morgan, Richard

AU - Schildkraut, Ira

AU - van Etten, James L.

N1 - Funding Information: ACKNOWLEDGEMENTS We thank Rich Roberts andStuart Linn for helpful discussions. The investigation was supported, in part, by Public Health Service grant GM-32441 from the National Institute of General Medicine and grant DE-ACO2-82ER12086 from the Department of Energy.

PY - 1988/10/25

Y1 - 1988/10/25

N2 - A site-specific endonuclease was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA-containing virus NYs-1. The enzyme recognizes the sequence 5'-CC-3' and cleaves 5' to the first C. It cleaves 5'-CmC-3' sequences but not 5'-mCC-3' sequences. The enzyme creates breaks in dsDNA whenever two 5'-CC-3' sequences on opposite strands are close enough for the two strands to separate; when the 5'-CC-3' sequences on opposite strands are further apart only a portion of the strands separate. Consequently, NYs-1 endonuclease does not produce a completely stable DNA digestion pattern. The enzyme probably does not cleave ssDNA and definitely does not cleave ssRNA or dsRNA.

AB - A site-specific endonuclease was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA-containing virus NYs-1. The enzyme recognizes the sequence 5'-CC-3' and cleaves 5' to the first C. It cleaves 5'-CmC-3' sequences but not 5'-mCC-3' sequences. The enzyme creates breaks in dsDNA whenever two 5'-CC-3' sequences on opposite strands are close enough for the two strands to separate; when the 5'-CC-3' sequences on opposite strands are further apart only a portion of the strands separate. Consequently, NYs-1 endonuclease does not produce a completely stable DNA digestion pattern. The enzyme probably does not cleave ssDNA and definitely does not cleave ssRNA or dsRNA.

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A site-specific single strand endonuclease activity induced by NYs-1 virus infection of a Chlorella-like green alga

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