A site-specific single strand endonuclease activity induced by NYs-1 virus infection of a Chlorella-like green alga

Yuannan Xia, Richard Morgan, Ira Schildkraut, James L. van Etten

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

A site-specific endonuclease was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA-containing virus NYs-1. The enzyme recognizes the sequence 5'-CC-3' and cleaves 5' to the first C. It cleaves 5'-CmC-3' sequences but not 5'-mCC-3' sequences. The enzyme creates breaks in dsDNA whenever two 5'-CC-3' sequences on opposite strands are close enough for the two strands to separate; when the 5'-CC-3' sequences on opposite strands are further apart only a portion of the strands separate. Consequently, NYs-1 endonuclease does not produce a completely stable DNA digestion pattern. The enzyme probably does not cleave ssDNA and definitely does not cleave ssRNA or dsRNA.

Original languageEnglish (US)
Pages (from-to)9477-9487
Number of pages11
JournalNucleic acids research
Volume16
Issue number20
DOIs
StatePublished - Oct 25 1988

ASJC Scopus subject areas

  • Genetics

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