A Smartphone App for Individual Xylazine/Ketamine Calculation Decreased Anesthesia-Related Mortality in Mice

Carlos Poblete Jara; Rodrigo S. Carraro; Ariane Zanesco; Beatriz Andrade; Karina Moreira; Guilherme Nogueira; Bruno L. Souza; Thais Paulino Prado; Valeria Póvoa; William Velander; Licio A. Velloso; Eliana P. Araújo

doi:10.3389/fvets.2021.651202

A Smartphone App for Individual Xylazine/Ketamine Calculation Decreased Anesthesia-Related Mortality in Mice

Carlos Poblete Jara, Rodrigo S. Carraro, Ariane Zanesco, Beatriz Andrade, Karina Moreira, Guilherme Nogueira, Bruno L. Souza, Thais Paulino Prado, Valeria Póvoa, William Velander, Licio A. Velloso, Eliana P. Araújo

Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Currently, experimental animals are widely used in biological and medical research. However, the scientific community has raised several bioethical concerns, such as the number of animals required to achieve reproducible and statistically relevant results. These concerns involve aspects related to pain, discomfort, and unwanted animal loss. Retrospectively, we compare two different approaches for anesthesia dosage: a mobile app for dose calculation and a standard dose calculation. A total of 939 C57BL/6J and Swiss mice were analyzed. We collected data on intraoperative and anesthesia-related mortality as described in electronic or physical handwritten records. Our results showed that the mobile app approach significantly reduces anesthetic-related deaths upon using doses of ketamine and xylazine. The results suggest that anesthesia-related mortality can be minimized even more using information technology approaches, helping to solve an old but transversal challenge for researchers working with experimental mice. The mobile app is a free and open code which could be implemented worldwide as an essential requirement for all anesthetic procedures in mice using xylazine and ketamine combination. As an open code app, the Labinsane initiative could also represent the starting point to unify and validate other anesthetic procedures in different species and strains.

Original language	English (US)
Article number	651202
Journal	Frontiers in Veterinary Science
Volume	8
DOIs	https://doi.org/10.3389/fvets.2021.651202
State	Published - Jul 22 2021

Keywords

anesthesia
mobile app
mortality and morbidity
smartphone
xylazine

ASJC Scopus subject areas

General Veterinary

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10.3389/fvets.2021.651202

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Jara, C. P., Carraro, R. S., Zanesco, A., Andrade, B., Moreira, K., Nogueira, G., Souza, B. L., Prado, T. P., Póvoa, V., Velander, W., Velloso, L. A., & Araújo, E. P. (2021). A Smartphone App for Individual Xylazine/Ketamine Calculation Decreased Anesthesia-Related Mortality in Mice. Frontiers in Veterinary Science, 8, Article 651202. https://doi.org/10.3389/fvets.2021.651202

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abstract = "Currently, experimental animals are widely used in biological and medical research. However, the scientific community has raised several bioethical concerns, such as the number of animals required to achieve reproducible and statistically relevant results. These concerns involve aspects related to pain, discomfort, and unwanted animal loss. Retrospectively, we compare two different approaches for anesthesia dosage: a mobile app for dose calculation and a standard dose calculation. A total of 939 C57BL/6J and Swiss mice were analyzed. We collected data on intraoperative and anesthesia-related mortality as described in electronic or physical handwritten records. Our results showed that the mobile app approach significantly reduces anesthetic-related deaths upon using doses of ketamine and xylazine. The results suggest that anesthesia-related mortality can be minimized even more using information technology approaches, helping to solve an old but transversal challenge for researchers working with experimental mice. The mobile app is a free and open code which could be implemented worldwide as an essential requirement for all anesthetic procedures in mice using xylazine and ketamine combination. As an open code app, the Labinsane initiative could also represent the starting point to unify and validate other anesthetic procedures in different species and strains.",

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author = "Jara, {Carlos Poblete} and Carraro, {Rodrigo S.} and Ariane Zanesco and Beatriz Andrade and Karina Moreira and Guilherme Nogueira and Souza, {Bruno L.} and Prado, {Thais Paulino} and Valeria P{\'o}voa and William Velander and Velloso, {Licio A.} and Ara{\'u}jo, {Eliana P.}",

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AU - Souza, Bruno L.

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AU - Velloso, Licio A.

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A Smartphone App for Individual Xylazine/Ketamine Calculation Decreased Anesthesia-Related Mortality in Mice

Abstract

Keywords

ASJC Scopus subject areas

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