A soft-agar procedure measuring growth of human colonic carcinomas

P. M. Kimball, M. G. Brattain, A. M. Pitts

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Cell suspensions from 5 human colonic carcinomas were fractionated by velocity sedimentation and plated in soft agar. Cluster formation was restricted to the purest fraction of epithelial cells, as had been determined by immuno- and histochemical criteria. Plating efficiencies for the 5 specimens were 1.0-4.5%. The effects of varying the incubation period and inoculum size upon growth were studied using unseparated cell suspensions from 6 specimens. Clusters were apparent after 3 weeks in culture, and maximum cluster formation was typically seen by 5 weeks. Cluster formation appeared concentration-dependent, and individual specimens varied with respect to the inoculum most conducive to growth. The maximum plating efficiencies for unseparated cells were unseparated cells were 0.4-1.7%.

Original languageEnglish (US)
Pages (from-to)1015-1019
Number of pages5
JournalBritish journal of cancer
Volume37
Issue number6
DOIs
StatePublished - Jun 1978

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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