Abstract
Cell suspensions from 5 human colonic carcinomas were fractionated by velocity sedimentation and plated in soft agar. Cluster formation was restricted to the purest fraction of epithelial cells, as had been determined by immuno- and histochemical criteria. Plating efficiencies for the 5 specimens were 1.0-4.5%. The effects of varying the incubation period and inoculum size upon growth were studied using unseparated cell suspensions from 6 specimens. Clusters were apparent after 3 weeks in culture, and maximum cluster formation was typically seen by 5 weeks. Cluster formation appeared concentration-dependent, and individual specimens varied with respect to the inoculum most conducive to growth. The maximum plating efficiencies for unseparated cells were unseparated cells were 0.4-1.7%.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1015-1019 |
| Number of pages | 5 |
| Journal | British journal of cancer |
| Volume | 37 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 1978 |
ASJC Scopus subject areas
- Oncology
- Cancer Research