A spectrum of HRT-dependent hypersensitive responses elicited by the 52 amino acid N-terminus of turnip crinkle virus capsid protein and its mutants

Sung Hwan Kang, Feng Qu, T. Jack Morris

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The capsid protein (CP) of turnip crinkle virus (TCV) is the elicitor of hypersensitive response (HR) and resistance mediated by the resistance protein HRT in the Di-17 ecotype of Arabidopsis. Here we identified the N-terminal 52-amino-acid R domain of TCV CP as the elicitor of HRT-dependent HR in Nicotiana benthamiana. Mutating this domain at position 6 (R6A), but not at positions 8 (R8A) or 14 (G14A), abolished HR in N. benthamiana. However, on Di-17 Arabidopsis leaves only R8A R domain elicited visible epidermal HR. When incorporated in infectious TCV RNAs, R8A and G14A mutations exerted dramatically different effects in Di-17 plants, as R8A caused systemic cell death whereas G14A led to complete restriction of the mutant virus. This continual spectrum of HR and resistance responses elicited by various R domain mutants suggests that the CP-HRT interaction could be perturbed by conformational changes in the R domain of TCV CP.

Original languageEnglish (US)
Pages (from-to)30-34
Number of pages5
JournalVirus Research
Volume200
Issue numberC
DOIs
StatePublished - Jan 1 2015

Keywords

  • Capsid protein
  • Hypersensitive response
  • Plant virus
  • Resistance protein
  • Turnip crinkle virus

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

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