A spirocyclohexyl nitroxide amino acid spin label for pulsed EPR spectroscopy distance measurements

Andrzej Rajca; Velavan Kathirvelu; Sandip K. Roy; Maren Pink; Suchada Rajca; Santanu Sarkar; Sandra S. Eaton; Gareth R. Eaton

doi:10.1002/chem.200903102

A spirocyclohexyl nitroxide amino acid spin label for pulsed EPR spectroscopy distance measurements

Andrzej Rajca, Velavan Kathirvelu, Sandip K. Roy, Maren Pink, Suchada Rajca, Santanu Sarkar, Sandra S. Eaton, Gareth R. Eaton

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

Site-directed spin labeling and EPR spectroscopy offer accurate, sensitive tools for the characterization of structure and function of macromolecules and their assemblies. A new rigid spin label, spirocyclohexyl nitroxide a-amino acid and its N-(9-fluorenylmethoxycarbonyl) derivative, have been synthesized, which exhibit slow enough spin-echo dephasing to permit accurate distance measurements by pulsed EPR spectroscopy at temperatures up to 125 K in 1:1 water/glycerol and at higher temperatures in matrices with higher glass transition temperatures. Distance measurements in the liquid nitrogen temperature range are less expensive than those that require liquid helium, which will greatly facilitate applications of pulsed EPR spectroscopy to the study of structure and conformation of peptides and proteins.

Original language	English (US)
Pages (from-to)	5778-5782
Number of pages	5
Journal	Chemistry - A European Journal
Volume	16
Issue number	19
DOIs	https://doi.org/10.1002/chem.200903102
State	Published - May 17 2010
Externally published	Yes

Keywords

EPR spectroscopy
Radicals
Spin labels spin relaxation
Spiro compounds

ASJC Scopus subject areas

Catalysis
Organic Chemistry

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10.1002/chem.200903102

Cite this

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title = "A spirocyclohexyl nitroxide amino acid spin label for pulsed EPR spectroscopy distance measurements",

abstract = "Site-directed spin labeling and EPR spectroscopy offer accurate, sensitive tools for the characterization of structure and function of macromolecules and their assemblies. A new rigid spin label, spirocyclohexyl nitroxide a-amino acid and its N-(9-fluorenylmethoxycarbonyl) derivative, have been synthesized, which exhibit slow enough spin-echo dephasing to permit accurate distance measurements by pulsed EPR spectroscopy at temperatures up to 125 K in 1:1 water/glycerol and at higher temperatures in matrices with higher glass transition temperatures. Distance measurements in the liquid nitrogen temperature range are less expensive than those that require liquid helium, which will greatly facilitate applications of pulsed EPR spectroscopy to the study of structure and conformation of peptides and proteins.",

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T1 - A spirocyclohexyl nitroxide amino acid spin label for pulsed EPR spectroscopy distance measurements

AU - Rajca, Andrzej

AU - Kathirvelu, Velavan

AU - Roy, Sandip K.

AU - Pink, Maren

AU - Rajca, Suchada

AU - Sarkar, Santanu

AU - Eaton, Sandra S.

AU - Eaton, Gareth R.

PY - 2010/5/17

Y1 - 2010/5/17

N2 - Site-directed spin labeling and EPR spectroscopy offer accurate, sensitive tools for the characterization of structure and function of macromolecules and their assemblies. A new rigid spin label, spirocyclohexyl nitroxide a-amino acid and its N-(9-fluorenylmethoxycarbonyl) derivative, have been synthesized, which exhibit slow enough spin-echo dephasing to permit accurate distance measurements by pulsed EPR spectroscopy at temperatures up to 125 K in 1:1 water/glycerol and at higher temperatures in matrices with higher glass transition temperatures. Distance measurements in the liquid nitrogen temperature range are less expensive than those that require liquid helium, which will greatly facilitate applications of pulsed EPR spectroscopy to the study of structure and conformation of peptides and proteins.

AB - Site-directed spin labeling and EPR spectroscopy offer accurate, sensitive tools for the characterization of structure and function of macromolecules and their assemblies. A new rigid spin label, spirocyclohexyl nitroxide a-amino acid and its N-(9-fluorenylmethoxycarbonyl) derivative, have been synthesized, which exhibit slow enough spin-echo dephasing to permit accurate distance measurements by pulsed EPR spectroscopy at temperatures up to 125 K in 1:1 water/glycerol and at higher temperatures in matrices with higher glass transition temperatures. Distance measurements in the liquid nitrogen temperature range are less expensive than those that require liquid helium, which will greatly facilitate applications of pulsed EPR spectroscopy to the study of structure and conformation of peptides and proteins.

KW - EPR spectroscopy

KW - Radicals

KW - Spin labels spin relaxation

KW - Spiro compounds

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A spirocyclohexyl nitroxide amino acid spin label for pulsed EPR spectroscopy distance measurements

Abstract

Keywords

ASJC Scopus subject areas

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