A ssDNA aptamer that blocks the function of the anti-FLAG M2 antibody

Amanda S. Lakamp, Michel M. Ouellette

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Using SELEX (systematic evolution of ligands by exponential enrichment), we serendipitously discovered a ssDNA aptamer that binds selectively to the anti-FLAG M2 antibody. The aptamer consisted of two motifs (CCTTA and TGTCTWCC) separated by 2-3 bases, and the elimination of one or the other motif abrogated binding. The DNA aptamer and FLAG peptide competed for binding to the antigen-binding pocket of the M2 antibody. In addition, the aptamer eluted FLAG-tagged proteins from the antibody, suggesting a commercial application in protein purification. These findings demonstrate the feasibility of using SELEX to develop ssDNA aptamers that block the function of a specific antibody, a capability that could lead to the development of novel therapeutic modalities for patients with systemic lupus erythematosus, rheumatoid arthritis, and other autoimmune diseases.

Original languageEnglish (US)
Article number720798
JournalJournal of Nucleic Acids
Volume2011
DOIs
StatePublished - 2011

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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