A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death

Jennifer L. Furman, Pui Wing Mok, Shengyi Shen, Cliff I. Stains, Indraneel Ghosh

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Designed sensors comprising split-firefly luciferase conjugated to tandem poly(ADP-ribose) binding domains allow for the direct solution phase detection of picogram quantities of PAR and for monitoring temporal changes in poly(ADP-ribosyl)ation events in mammalian cells.

Original languageEnglish (US)
Pages (from-to)397-399
Number of pages3
JournalChemical Communications
Volume47
Issue number1
DOIs
StatePublished - Dec 6 2011
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Fingerprint Dive into the research topics of 'A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death'. Together they form a unique fingerprint.

  • Cite this