A biosensor based on tyrosinase as an expanded bed reactor was developed for the determination of phenolic compounds. The expanded bed mode of the analytical unit allows the analysis of samples containing particulate matter. Linear calibration graphs were obtained for both phenol and catechol up to 100 μM. At a flow rate of 0.5 ml/min, the peak maximum was obtained within 5 min and is highly responsive especially towards phenol, catechol and cresol. Repetitive injections of catechol and phenol have yielded no obvious variation in response indicating the operational feasibility of the sensor. The expanded bed mode enzyme sensor for phenol provides a means for the real time monitoring of environmental bioreactors.
|Number of pages
|Resource and Environmental Biotechnology
|Published - 1999
ASJC Scopus subject areas
- General Environmental Science