A vancomycin photoprobe identifies the histidine kinase VanSsc as a vancomycin receptor

Kalinka Koteva, Hee Jeon Hong, Xiao Dong Wang, Ishac Nazi, Donald Hughes, Mike J. Naldrett, Mark J. Buttner, Gerard D. Wright

Research output: Contribution to journalArticlepeer-review

97 Scopus citations

Abstract

Inducible resistance to the glycopeptide antibiotic vancomycin requires expression of vanH, vanA and vanX, controlled by a two-component regulatory system consisting of a receptor histidine kinase, VanS, and a response regulator, VanR. The identity of the VanS receptor ligand has been debated. Using a synthesized vancomycin photoaffinity probe, we show that vancomycin directly binds Streptomyces coelicolor VanS (VanSsc) and this binding is correlated with resistance and required for vanH, vanA and vanX gene expression.

Original languageEnglish (US)
Pages (from-to)327-329
Number of pages3
JournalNature Chemical Biology
Volume6
Issue number5
DOIs
StatePublished - May 2010
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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