Aberrant expression of microRNAs in gastric cancer and biological significance of miR-574-3p

Yingying Su; Zhaohui Ni; Guoqing Wang; Juan Cui; Chengguo Wei; Jihan Wang; Qing Yang; Ying Xu; Fan Li

doi:10.1016/j.intimp.2012.05.016

Aberrant expression of microRNAs in gastric cancer and biological significance of miR-574-3p

Yingying Su, Zhaohui Ni, Guoqing Wang, Juan Cui, Chengguo Wei, Jihan Wang, Qing Yang, Ying Xu, Fan Li

Research output: Contribution to journal › Article › peer-review

66 Scopus citations

Abstract

The discovery of microRNAs (miRNAs) provides a new and powerful tool for studying the mechanisms, diagnosis and treatments of cancer. In this study, we employed AFFX miRNA expression chips to search for miRNAs that may be aberrantly expressed in gastric cancer tissues and to investigate the potential roles that miRNAs may play in the development and progression of gastric cancer. 14 miRNAs were found to be down-regulated and 2 miRNAs up-regulated in gastric cancer tissues compared to the normal gastric tissues. Among the aberrantly expressed miRNAs, miR-574-3p was selected to further study its expression features and functional roles. Interestingly, the reduced expression of miR-574-3p occurred mainly in the early stages of gastric cancer or in cancers with high level of differentiation, suggesting that it can be used as a marker for a mild case of gastric cancer. Functional study revealed that cell proliferation, migration and invasion were significantly inhibited in miR-574-3p-transfected gastric cancer SGC7901 cells. Computational prediction and experimental validation suggest that Cullin2 may be one of the targets of miR-574-3p. Overall our study suggests that the aberrantly expressed miRNAs may play regulatory and functional roles in the development and progression of gastric cancer.

Original language	English (US)
Pages (from-to)	468-475
Number of pages	8
Journal	International Immunopharmacology
Volume	13
Issue number	4
DOIs	https://doi.org/10.1016/j.intimp.2012.05.016
State	Published - Aug 2012
Externally published	Yes

Keywords

Gastric cancer
Invasion
MicroRNA
Proliferation
Target gene

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Pharmacology

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10.1016/j.intimp.2012.05.016

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@article{6a2b8da7e214402b931870ac34fd27a6,

title = "Aberrant expression of microRNAs in gastric cancer and biological significance of miR-574-3p",

abstract = "The discovery of microRNAs (miRNAs) provides a new and powerful tool for studying the mechanisms, diagnosis and treatments of cancer. In this study, we employed AFFX miRNA expression chips to search for miRNAs that may be aberrantly expressed in gastric cancer tissues and to investigate the potential roles that miRNAs may play in the development and progression of gastric cancer. 14 miRNAs were found to be down-regulated and 2 miRNAs up-regulated in gastric cancer tissues compared to the normal gastric tissues. Among the aberrantly expressed miRNAs, miR-574-3p was selected to further study its expression features and functional roles. Interestingly, the reduced expression of miR-574-3p occurred mainly in the early stages of gastric cancer or in cancers with high level of differentiation, suggesting that it can be used as a marker for a mild case of gastric cancer. Functional study revealed that cell proliferation, migration and invasion were significantly inhibited in miR-574-3p-transfected gastric cancer SGC7901 cells. Computational prediction and experimental validation suggest that Cullin2 may be one of the targets of miR-574-3p. Overall our study suggests that the aberrantly expressed miRNAs may play regulatory and functional roles in the development and progression of gastric cancer.",

keywords = "Gastric cancer, Invasion, MicroRNA, Proliferation, Target gene",

author = "Yingying Su and Zhaohui Ni and Guoqing Wang and Juan Cui and Chengguo Wei and Jihan Wang and Qing Yang and Ying Xu and Fan Li",

note = "Funding Information: We thank the physicians in the First Hospital of Jilin University for providing the specimens used in this study. We also thank Dr. Ming Yang, Department of Immunological Laboratory, Jilin University, for his technical help. This work was supported in part by the National Institutes of Health ( 1R01GM075331 ), a “Distinguished Scholar” grant from the Georgia Cancer Coalition , and seed funding from the University of Georgia and in part by Research Fund for the Innovation Program of Higher Education of China and National Basic Research Program of China (973 program, 2011CB512003 ) and National Natural Science Foundation of China ( 30872415 and 81071424 ). ",

year = "2012",

month = aug,

doi = "10.1016/j.intimp.2012.05.016",

language = "English (US)",

volume = "13",

pages = "468--475",

journal = "International Immunopharmacology",

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T1 - Aberrant expression of microRNAs in gastric cancer and biological significance of miR-574-3p

AU - Su, Yingying

AU - Ni, Zhaohui

AU - Wang, Guoqing

AU - Cui, Juan

AU - Wei, Chengguo

AU - Wang, Jihan

AU - Yang, Qing

AU - Xu, Ying

AU - Li, Fan

N1 - Funding Information: We thank the physicians in the First Hospital of Jilin University for providing the specimens used in this study. We also thank Dr. Ming Yang, Department of Immunological Laboratory, Jilin University, for his technical help. This work was supported in part by the National Institutes of Health ( 1R01GM075331 ), a “Distinguished Scholar” grant from the Georgia Cancer Coalition , and seed funding from the University of Georgia and in part by Research Fund for the Innovation Program of Higher Education of China and National Basic Research Program of China (973 program, 2011CB512003 ) and National Natural Science Foundation of China ( 30872415 and 81071424 ).

PY - 2012/8

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N2 - The discovery of microRNAs (miRNAs) provides a new and powerful tool for studying the mechanisms, diagnosis and treatments of cancer. In this study, we employed AFFX miRNA expression chips to search for miRNAs that may be aberrantly expressed in gastric cancer tissues and to investigate the potential roles that miRNAs may play in the development and progression of gastric cancer. 14 miRNAs were found to be down-regulated and 2 miRNAs up-regulated in gastric cancer tissues compared to the normal gastric tissues. Among the aberrantly expressed miRNAs, miR-574-3p was selected to further study its expression features and functional roles. Interestingly, the reduced expression of miR-574-3p occurred mainly in the early stages of gastric cancer or in cancers with high level of differentiation, suggesting that it can be used as a marker for a mild case of gastric cancer. Functional study revealed that cell proliferation, migration and invasion were significantly inhibited in miR-574-3p-transfected gastric cancer SGC7901 cells. Computational prediction and experimental validation suggest that Cullin2 may be one of the targets of miR-574-3p. Overall our study suggests that the aberrantly expressed miRNAs may play regulatory and functional roles in the development and progression of gastric cancer.

AB - The discovery of microRNAs (miRNAs) provides a new and powerful tool for studying the mechanisms, diagnosis and treatments of cancer. In this study, we employed AFFX miRNA expression chips to search for miRNAs that may be aberrantly expressed in gastric cancer tissues and to investigate the potential roles that miRNAs may play in the development and progression of gastric cancer. 14 miRNAs were found to be down-regulated and 2 miRNAs up-regulated in gastric cancer tissues compared to the normal gastric tissues. Among the aberrantly expressed miRNAs, miR-574-3p was selected to further study its expression features and functional roles. Interestingly, the reduced expression of miR-574-3p occurred mainly in the early stages of gastric cancer or in cancers with high level of differentiation, suggesting that it can be used as a marker for a mild case of gastric cancer. Functional study revealed that cell proliferation, migration and invasion were significantly inhibited in miR-574-3p-transfected gastric cancer SGC7901 cells. Computational prediction and experimental validation suggest that Cullin2 may be one of the targets of miR-574-3p. Overall our study suggests that the aberrantly expressed miRNAs may play regulatory and functional roles in the development and progression of gastric cancer.

KW - Gastric cancer

KW - Invasion

KW - MicroRNA

KW - Proliferation

KW - Target gene

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Aberrant expression of microRNAs in gastric cancer and biological significance of miR-574-3p

Abstract

Keywords

ASJC Scopus subject areas

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