TY - JOUR
T1 - Abnormal Accumulation of Desmin in Gastrocnemius Myofibers of Patients with Peripheral Artery Disease
T2 - Associations with Altered Myofiber Morphology and Density, Mitochondrial Dysfunction and Impaired Limb Function
AU - Koutakis, Panagiotis
AU - Miserlis, Dimitrios
AU - Myers, Sara A.
AU - Kim, Julian Kyung Soo
AU - Zhu, Zhen
AU - Papoutsi, Evlampia
AU - Swanson, Stanley A.
AU - Haynatzki, Gleb
AU - Ha, Duy M.
AU - Carpenter, Lauren A.
AU - McComb, Rodney D.
AU - Johanning, Jason M.
AU - Casale, George P.
AU - Pipinos, Iraklis I.I.
N1 - Publisher Copyright:
© The Author(s) 2015.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - Patients with peripheral artery disease (PAD) develop a myopathy in their ischemic lower extremities, which is characterized by myofiber degeneration, mitochondrial dysfunction and impaired limb function. Desmin, a protein of the cytoskeleton, is central to maintenance of the structure, shape and function of the myofiber and its organelles, especially the mitochondria, and to translation of sarcomere contraction into muscle contraction. In this study, we investigated the hypothesis that disruption of the desmin network occurs in gastrocnemius myofibers of PAD patients and correlates with altered myofiber morphology, mitochondrial dysfunction, and impaired limb function. Using fluorescence microscopy, we evaluated desmin organization and quantified myofiber content in the gastrocnemius of PAD and control patients. Desmin was highly disorganized in PAD but not control muscles and myofiber content was increased significantly in PAD compared to control muscles. By qPCR, we found that desmin gene transcripts were increased in the gastrocnemius of PAD patients as compared with control patients. Increased desmin and desmin gene transcripts in PAD muscles correlated with altered myofiber morphology, decreased mitochondrial respiration, reduced calf muscle strength and decreased walking performance. In conclusion, our studies identified disruption of the desmin system in gastrocnemius myofibers as an index of the myopathy and limitation of muscle function in patients with PAD.
AB - Patients with peripheral artery disease (PAD) develop a myopathy in their ischemic lower extremities, which is characterized by myofiber degeneration, mitochondrial dysfunction and impaired limb function. Desmin, a protein of the cytoskeleton, is central to maintenance of the structure, shape and function of the myofiber and its organelles, especially the mitochondria, and to translation of sarcomere contraction into muscle contraction. In this study, we investigated the hypothesis that disruption of the desmin network occurs in gastrocnemius myofibers of PAD patients and correlates with altered myofiber morphology, mitochondrial dysfunction, and impaired limb function. Using fluorescence microscopy, we evaluated desmin organization and quantified myofiber content in the gastrocnemius of PAD and control patients. Desmin was highly disorganized in PAD but not control muscles and myofiber content was increased significantly in PAD compared to control muscles. By qPCR, we found that desmin gene transcripts were increased in the gastrocnemius of PAD patients as compared with control patients. Increased desmin and desmin gene transcripts in PAD muscles correlated with altered myofiber morphology, decreased mitochondrial respiration, reduced calf muscle strength and decreased walking performance. In conclusion, our studies identified disruption of the desmin system in gastrocnemius myofibers as an index of the myopathy and limitation of muscle function in patients with PAD.
KW - Cytoskeleton
KW - desmin
KW - intermittent claudication
KW - muscle disease
KW - myofiber
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U2 - 10.1369/0022155415569348
DO - 10.1369/0022155415569348
M3 - Article
C2 - 25575565
AN - SCOPUS:84925616262
SN - 0022-1554
VL - 63
SP - 256
EP - 269
JO - Journal of Histochemistry and Cytochemistry
JF - Journal of Histochemistry and Cytochemistry
IS - 4
ER -