TY - JOUR
T1 - Abnormal myofiber morphology and limb dysfunction in claudication
AU - Koutakis, Panagiotis
AU - Myers, Sara A.
AU - Cluff, Kim
AU - Ha, Duy M.
AU - Haynatzki, Gleb
AU - McComb, Rodney D.
AU - Uchida, Koji
AU - Miserlis, Dimitrios
AU - Papoutsi, Evlampia
AU - Johanning, Jason M.
AU - Casale, George P.
AU - Pipinos, Iraklis I.
N1 - Funding Information:
This work was primarily supported by NIH grant R01AG034995 , by the Charles and Mary Heider Fund for Excellence in Vascular Surgery, by the Alexander S. Onassis Public Benefit Foundation F-ZD036 , and by the American Heart Association Pre-Doctoral Fellowship 13PRE13860010. Secondary funding was provided by the Department of Veterans' Affairs, Veterans Health Administration, Rehabilitation Research, and Development Service 1I01RX000604 and by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number P20GM109090. The content of this paper is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Furthermore, this material is the result of work supported with resources and the use of facilities at the VA Nebraska-Western Iowa Health Care System.
Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - Background Peripheral artery disease (PAD), which affects an estimated 27 million people in Europe and North America, is caused by atherosclerotic plaques that limit blood flow to the legs. Chronic, repeated ischemia in the lower leg muscles of PAD patients is associated with loss of normal myofiber morphology and myofiber degradation. In this study, we tested the hypothesis that myofiber morphometrics of PAD calf muscle are significantly different from normal calf muscle and correlate with reduced calf muscle strength and walking performance. Methods Gastrocnemius biopsies were collected from 154 PAD patients (Fontaine stage II) and 85 control subjects. Morphometric parameters of gastrocnemius fibers were determined and evaluated for associations with walking distances and calf muscle strength. Results Compared with control myofibers, PAD myofiber cross-sectional area, major and minor axes, equivalent diameter, perimeter, solidity, and density were significantly decreased (P < 0.005), whereas roundness was significantly increased (P < 0.005). Myofiber morphometric parameters correlated with walking distances and calf muscle strength. Multiple regression analyses demonstrated myofiber cross-sectional area, roundness, and solidity as the best predictors of calf muscle strength and 6-min walking distance, whereas cross-sectional area was the main predictor of maximum walking distance. Conclusions Myofiber morphometrics of PAD gastrocnemius differ significantly from those of control muscle and predict calf muscle strength and walking distances of the PAD patients. Morphometric parameters of gastrocnemius myofibers may serve as objective criteria for diagnosis, staging, and treatment of PAD.
AB - Background Peripheral artery disease (PAD), which affects an estimated 27 million people in Europe and North America, is caused by atherosclerotic plaques that limit blood flow to the legs. Chronic, repeated ischemia in the lower leg muscles of PAD patients is associated with loss of normal myofiber morphology and myofiber degradation. In this study, we tested the hypothesis that myofiber morphometrics of PAD calf muscle are significantly different from normal calf muscle and correlate with reduced calf muscle strength and walking performance. Methods Gastrocnemius biopsies were collected from 154 PAD patients (Fontaine stage II) and 85 control subjects. Morphometric parameters of gastrocnemius fibers were determined and evaluated for associations with walking distances and calf muscle strength. Results Compared with control myofibers, PAD myofiber cross-sectional area, major and minor axes, equivalent diameter, perimeter, solidity, and density were significantly decreased (P < 0.005), whereas roundness was significantly increased (P < 0.005). Myofiber morphometric parameters correlated with walking distances and calf muscle strength. Multiple regression analyses demonstrated myofiber cross-sectional area, roundness, and solidity as the best predictors of calf muscle strength and 6-min walking distance, whereas cross-sectional area was the main predictor of maximum walking distance. Conclusions Myofiber morphometrics of PAD gastrocnemius differ significantly from those of control muscle and predict calf muscle strength and walking distances of the PAD patients. Morphometric parameters of gastrocnemius myofibers may serve as objective criteria for diagnosis, staging, and treatment of PAD.
KW - Claudication
KW - Myofiber morphology
KW - Walking distance
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U2 - 10.1016/j.jss.2015.02.011
DO - 10.1016/j.jss.2015.02.011
M3 - Article
C2 - 25791828
AN - SCOPUS:84929274039
VL - 196
SP - 172
EP - 179
JO - Journal of Surgical Research
JF - Journal of Surgical Research
SN - 0022-4804
IS - 1
ER -