Absolute blood velocity measured with a modified fundus camera

Donald D. Duncan, Paul Lemaillet, Mohamed Ibrahim, Quan Dong Nguyen, Matthias Hiller, Jessica Ramella-Roman

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


We present a new method for the quantitative estimation of blood flow velocity, based on the use of the Radon transform. The specific application is for measurement of blood flow velocity in the retina. Our modified fundus camera uses illumination from a green LED and captures imagery with a high-speed CCD camera. The basic theory is presented, and typical results are shown for an in vitro flow model using blood in a capillary tube. Subsequently, representative results are shown for representative fundus imagery. This approach provides absolute velocity and flow direction along the vessel center-line or any lateral displacement therefrom. We also provide an error analysis allowing estimation of confidence intervals for the estimated velocity.

Original languageEnglish (US)
Article number056014
JournalJournal of Biomedical Optics
Issue number5
StatePublished - Sep 2010
Externally publishedYes


  • Absolute blood velocity
  • Flow
  • Perfusion
  • Radon transform

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering


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