Acrosome reaction is subfamily specific in sea star fertilization

Mia Nakachi, Hideaki Moriyama, Motonori Hoshi, Midori Matsumoto

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

In the fertilization process of sea stars, sperm is activated to go through the acrosome reaction before cell fusion. We focused on induction of the acrosome reaction as a key process in fertilization. Six species of sea stars were used in this study: Asterias amurensis, Asterias rubens, Asterias forbesi, Aphelasterias japonica, Distolasterias nipon, and Asterina pectinifera. Acrosome reaction assays indicate that the acrosome reaction can be induced across species within Asteriinae subfamily. However, cross-fertilization assays indicate that sea stars have species specificity in fertilization. Therefore, steps after the acrosome reaction are responsible for the species specificity. To explain acrosome reaction subfamily specificity at the molecular level, the sugar components of egg jelly were examined and analyzed by principal component analysis. A. amurensis and A. forbesi belong to the same induction group of the acrosome reaction. D. nipon and An. pectinifera are in a unique group. Enzyme-linked immunosorbent assays indicate that Asteriinae subfamily share a common glycan structure, the Fragment 1 of Acrosome Reaction-Inducing Substance from A. amurensis. Fragment 1 plays an important role in the subfamily specificity of acrosome reaction induction. In addition, A. amurensis sperm activating peptide was recognized by sperm from the same superorder. These results demonstrate that the specificity of acrosome reaction induction is present at the subfamily level in sea stars.

Original languageEnglish (US)
Pages (from-to)597-604
Number of pages8
JournalDevelopmental Biology
Volume298
Issue number2
DOIs
StatePublished - Oct 15 2006

Keywords

  • Acrosome reaction
  • Aphelasterias japonica
  • Asterias amurensis
  • Asterias forbesi
  • Asterias rubens
  • Asterina pectinifera
  • Distolasterias nipon
  • Egg jelly
  • Fertilization
  • Species specificity

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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