Activation of a COI1-dependent pathway in Arabidopsis by Pseudomonas syringae type III effectors and coronatine

Ping He, Satya Chintamanani, Zhongying Chen, Lihuang Zhu, Barbara N. Kunkel, James R. Alfano, Xiaoyan Tang, Jian Min Zhou

Research output: Contribution to journalArticle

106 Scopus citations

Abstract

Gram-negative bacteria use a variety of virulence factors including phytotoxins, exopolysaccharides, effectors secreted by the type III secretion system, and cell-wall-degrading enzymes to promote parasitism in plants. However, little is known about how these virulence factors alter plant cellular responses to promote disease. In this study, we show that virulent Pseudomonas syringae strains activate the transcription of an Arabidopsis ethylene response factor (ERF) gene, RAP2.6, in a coronatine insensitive 1 (COI1)-dependent manner. A highly sensitive RAP2.6 promoter-firefly luciferase (RAP2.6-LUC) reporter line was developed to monitor activities of various bacterial virulence genes. Analyses of P. syringae pv. tomato DC3000 mutants indicated that both type III secretion system and the phytotoxin coronatine are required for RAP2.6 induction. We show that at least five individual type III effectors, avirulence B (AvrB), AvrRpt2, AvrPphB, HopPtoK, and AvrPphEPto, contributed to RAP2.6 induction. Gene-for-gene recognition was not involved in RAP2.6 induction because plants lacking RPM1 and RPS2 responded normally to AvrB and AvrRpt2 in RAP2.6 expression. Interestingly, the role of coronatine in RAP2.6 induction can be partially substituted by the addition of avrB in DC3000, suggesting that AvrB may mimic coronatine. These results suggest that P. syringae type III effectors and coronatine act by augmenting a COI1-dependent pathway to promote parasitism.

Original languageEnglish (US)
Pages (from-to)589-602
Number of pages14
JournalPlant Journal
Volume37
Issue number4
DOIs
StatePublished - Feb 2004

Keywords

  • Coronatine
  • ERF
  • Jasmonates
  • Pseudomonas
  • Type III effectors
  • Virulence

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

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