Activation of LEDGF gene by thermal- and oxidative-stresses

Preeti Sharma, Dhirendra P. Singh, Nigar Fatma, Leo T. Chylack, Toshimichi Shinohara

Research output: Contribution to journalArticle

58 Scopus citations

Abstract

LEDGF promotes survival of many cell types against a wide range of environmental stresses, and cells responding to those stresses expressed higher levels of heat shock proteins (Hsps). LEDGF/p75 is a weak coactivator of general transcription. We speculated that a stress signal may activate expression of the LEDGF gene, resulting in elevated levels of LEDGF which may activate expression of stress-related proteins to protect cells from stresses. Lens epithelial cells (LECs) and cos7 cells were cultured under heat- or oxidative-stress. After cells were cultured for defined time, we quantified LEDGF mRNA and LEDGF protein with RT-PCR, Northern blot, and protein blot analysis. Our results showed that higher levels of LEDGF were found in both cell types under heat- and oxidative-stress than that in cells cultured at nonstress condition. Thus, one of the primary events in the stress-related protective events is the activation of LEDGF, a regulatory protein. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)1320-1324
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume276
Issue number3
DOIs
StatePublished - Oct 5 2000

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Keywords

  • Gene activation
  • Heat shock-stress
  • Lens epithelium-derived growth factor (LEDGF)
  • Oxidative-stress
  • Survival factor

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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