Activation of recombinant human protein C

Timothy K. Lee; Neelesh Bangalore; William Velander; William N. Drohan; Henryk Lubon

doi:10.1016/0049-3848(96)00069-2

Activation of recombinant human protein C

Timothy K. Lee, Neelesh Bangalore, William Velander, William N. Drohan, Henryk Lubon

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

We have produced recombinant human Protein C (rHPC) in the milk of transgenic swine. After purification, we have analyzed the interaction of the zymogen with Protac, thrombin/thrombomodulin and thrombin alone. The amidolytic and anticoagulant activities of rAPC after Protac activation were ~80% those of its human plasma counterpart. Upon the excision of the activation peptide by thrombin/thrombomodulin complex, both the natural and recombinant activation products had similar enzymatic and biological activities. This. observation can be attributed to the difference in the mechanism of action between the two activators and structural differences between HPC and rHPC.

Original language	English (US)
Pages (from-to)	225-234
Number of pages	10
Journal	Thrombosis Research
Volume	82
Issue number	3
DOIs	https://doi.org/10.1016/0049-3848(96)00069-2
State	Published - May 1 1996
Externally published	Yes

Keywords

Protac
Protein C
Thrombin
Thrombomodulin
Transgenic pig

ASJC Scopus subject areas

Hematology

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10.1016/0049-3848(96)00069-2

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title = "Activation of recombinant human protein C",

abstract = "We have produced recombinant human Protein C (rHPC) in the milk of transgenic swine. After purification, we have analyzed the interaction of the zymogen with Protac, thrombin/thrombomodulin and thrombin alone. The amidolytic and anticoagulant activities of rAPC after Protac activation were ~80% those of its human plasma counterpart. Upon the excision of the activation peptide by thrombin/thrombomodulin complex, both the natural and recombinant activation products had similar enzymatic and biological activities. This. observation can be attributed to the difference in the mechanism of action between the two activators and structural differences between HPC and rHPC.",

keywords = "Protac, Protein C, Thrombin, Thrombomodulin, Transgenic pig",

author = "Lee, {Timothy K.} and Neelesh Bangalore and William Velander and Drohan, {William N.} and Henryk Lubon",

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doi = "10.1016/0049-3848(96)00069-2",

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TY - JOUR

T1 - Activation of recombinant human protein C

AU - Lee, Timothy K.

AU - Bangalore, Neelesh

AU - Velander, William

AU - Drohan, William N.

AU - Lubon, Henryk

PY - 1996/5/1

Y1 - 1996/5/1

N2 - We have produced recombinant human Protein C (rHPC) in the milk of transgenic swine. After purification, we have analyzed the interaction of the zymogen with Protac, thrombin/thrombomodulin and thrombin alone. The amidolytic and anticoagulant activities of rAPC after Protac activation were ~80% those of its human plasma counterpart. Upon the excision of the activation peptide by thrombin/thrombomodulin complex, both the natural and recombinant activation products had similar enzymatic and biological activities. This. observation can be attributed to the difference in the mechanism of action between the two activators and structural differences between HPC and rHPC.

AB - We have produced recombinant human Protein C (rHPC) in the milk of transgenic swine. After purification, we have analyzed the interaction of the zymogen with Protac, thrombin/thrombomodulin and thrombin alone. The amidolytic and anticoagulant activities of rAPC after Protac activation were ~80% those of its human plasma counterpart. Upon the excision of the activation peptide by thrombin/thrombomodulin complex, both the natural and recombinant activation products had similar enzymatic and biological activities. This. observation can be attributed to the difference in the mechanism of action between the two activators and structural differences between HPC and rHPC.

KW - Protac

KW - Protein C

KW - Thrombin

KW - Thrombomodulin

KW - Transgenic pig

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DO - 10.1016/0049-3848(96)00069-2

M3 - Article

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AN - SCOPUS:0030014889

SN - 0049-3848

VL - 82

SP - 225

EP - 234

JO - Thrombosis Research

JF - Thrombosis Research

IS - 3

ER -

Activation of recombinant human protein C

Abstract

Keywords

ASJC Scopus subject areas

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