Activation of recombinant human protein C

Timothy K. Lee, Neelesh Bangalore, William Velander, William N. Drohan, Henryk Lubon

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

We have produced recombinant human Protein C (rHPC) in the milk of transgenic swine. After purification, we have analyzed the interaction of the zymogen with Protac, thrombin/thrombomodulin and thrombin alone. The amidolytic and anticoagulant activities of rAPC after Protac activation were ~80% those of its human plasma counterpart. Upon the excision of the activation peptide by thrombin/thrombomodulin complex, both the natural and recombinant activation products had similar enzymatic and biological activities. This. observation can be attributed to the difference in the mechanism of action between the two activators and structural differences between HPC and rHPC.

Original languageEnglish (US)
Pages (from-to)225-234
Number of pages10
JournalThrombosis Research
Volume82
Issue number3
DOIs
StatePublished - May 1 1996

Keywords

  • Protac
  • Protein C
  • Thrombin
  • Thrombomodulin
  • Transgenic pig

ASJC Scopus subject areas

  • Hematology

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    Lee, T. K., Bangalore, N., Velander, W., Drohan, W. N., & Lubon, H. (1996). Activation of recombinant human protein C. Thrombosis Research, 82(3), 225-234. https://doi.org/10.1016/0049-3848(96)00069-2