Active Ebola Virus Replication and Heterogeneous Evolutionary Rates in EVD Survivors

Ebola Virus Persistence Study Group

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Following cessation of continuous Ebola virus (EBOV) transmission within Western Africa, sporadic EBOV disease (EVD) cases continued to re-emerge beyond the viral incubation period. Epidemiological and genomic evidence strongly suggests that this represented transmission from EVD survivors. To investigate whether persistent infections are characterized by ongoing viral replication, we sequenced EBOV from the semen of nine EVD survivors and a subset of corresponding acute specimens. EBOV evolutionary rates during persistence were either similar to or reduced relative to acute infection rates. Active EBOV replication/transcription continued during convalescence, but decreased over time, consistent with viral persistence rather than viral latency. Patterns of genetic divergence suggest a moderate relaxation of selective constraints within the sGP carboxy-terminal tail during persistent infections, but do not support widespread diversifying selection. Altogether, our data illustrate that EBOV persistence in semen, urine, and aqueous humor is not a quiescent or latent infection. Whitmer et al. find that Ebola virus continues replication/transcription within the eye and male genital tract of Ebola virus disease survivors. They describe viral replication, evolutionary rates, and selective pressures experienced during acute and persistent infection.

Original languageEnglish (US)
Pages (from-to)1159-1168
Number of pages10
JournalCell Reports
Volume22
Issue number5
DOIs
StatePublished - Jan 30 2018

Keywords

  • EVD survivors
  • Ebola virus
  • RNA hyper-editing
  • evolutionary pressure
  • evolutionary rates
  • persistent viral infection

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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