Acute high-intensity exercise impairs skeletal muscle respiratory capacity

Gwenael Layec, Gregory M. Blain, Matthew J. Rossman, Song Y. Park, Corey R. Hart, Joel D. Trinity, Jayson R. Gifford, Simranjit K. Sidhu, Joshua C. Weavil, Thomas J. Hureau, Markus Amann, Russell S. Richardson

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Purpose The effect of an acute bout of exercise, especially high-intensity exercise, on the function of mitochondrial respiratory complexes is not well understood, with potential implications for both the healthy population and patients undergoing exercise-based rehabilitation. Therefore, this study sought to comprehensively examine respiratory flux through the different complexes of the electron transport chain in skeletal muscle mitochondria before and immediately after high-intensity aerobic exercise. Methods Muscle biopsies of the vastus lateralis were obtained at baseline and immediately after a 5-km time trial performed on a cycle ergometer. Mitochondrial respiratory flux through the complexes of the electron transport chain was measured in permeabilized skeletal muscle fibers by high-resolution respirometry. Results Complex I + II state 3 (state 3CI + CII) respiration, a measure of oxidative phosphorylation capacity, was diminished immediately after the exercise (pre, 27 ± 3 ρm·mg-1·s-1; post, 17 ± 2 ρm·mg-1·s-1; P < 0.05). This decreased oxidative phosphorylation capacity was predominantly the consequence of attenuated complex II-driven state 3 (state 3CII) respiration (pre, 17 ± 1 ρm·mg-1·s-1; post, 9 ± 2 ρm·mg-1·s-1; P < 0.05). Although complex I-driven state 3 (3CI) respiration was also lower (pre, 20 ± 2 ρm·mg-1·s-1; post, 14 ± 4 ρm·mg-1·s-1), this did not reach statistical significance (P = 0.27). In contrast, citrate synthase activity, proton leak (state 2 respiration), and complex IV capacity were not significantly altered immediately after the exercise. Conclusions These findings reveal that acute high-intensity aerobic exercise significantly inhibits skeletal muscle state 3CII and oxidative phosphorylation capacity. This, likely transient, mitochondrial defect might amplify the exercise-induced development of fatigue and play an important role in initiating exercise-induced mitochondrial adaptations.

Original languageEnglish (US)
Pages (from-to)2409-2417
Number of pages9
JournalMedicine and science in sports and exercise
Volume50
Issue number12
DOIs
StatePublished - Dec 1 2018

Keywords

  • CYCLING TIME TRIAL
  • ELECTRON TRANSPORT CHAIN
  • MITOHORMESIS
  • OXIDATIVE PHOSPHORYLATION CAPACITY
  • STATE 3 RESPIRATION

ASJC Scopus subject areas

  • Physical Therapy, Sports Therapy and Rehabilitation
  • Orthopedics and Sports Medicine

Fingerprint

Dive into the research topics of 'Acute high-intensity exercise impairs skeletal muscle respiratory capacity'. Together they form a unique fingerprint.

Cite this