Addition of an α-hydroxy acid to the genetic code of bacteria

Jiantao Guo; Jiangyun Wang; J. Christopher Anderson; Peter G. Schultz

doi:10.1002/anie.200704074

Addition of an α-hydroxy acid to the genetic code of bacteria

Jiantao Guo, Jiangyun Wang, J. Christopher Anderson, Peter G. Schultz

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

(Chemical Equation Presented) Playing tag: The α-hydroxy acid p-hydroxy-L-phenyllactic acid has been genetically incorporated into proteins in E. coli in response to an amber nonsense codon. The site-specific introduction of backbone ester mutations was used for the site-specific hydrolysis of an affinity tag, as well as for determination of the energetic contributions of backbone hydrogen bonds to protein stability.

Original language	English (US)
Pages (from-to)	722-725
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	47
Issue number	4
DOIs	https://doi.org/10.1002/anie.200704074
State	Published - 2008
Externally published	Yes

Keywords

Backbone mutations
Biosynthesis
Proteins
α-hydroxy acids

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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abstract = "(Chemical Equation Presented) Playing tag: The α-hydroxy acid p-hydroxy-L-phenyllactic acid has been genetically incorporated into proteins in E. coli in response to an amber nonsense codon. The site-specific introduction of backbone ester mutations was used for the site-specific hydrolysis of an affinity tag, as well as for determination of the energetic contributions of backbone hydrogen bonds to protein stability.",

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