Adenosine uptake sites in rat brain: Identification using [3H]nitrobenzylthioinosine and co-localization with adenosine deaminase

J. I. Nagy; J. D. Geiger; P. E. Daddona

doi:10.1016/0304-3940(85)90310-6

Adenosine uptake sites in rat brain: Identification using [³H]nitrobenzylthioinosine and co-localization with adenosine deaminase

J. I. Nagy, J. D. Geiger, P. E. Daddona

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

The binding characteristics of [³H]nitrobenzylthioinosine ([³H]NBI) to rat brain membrane preparations was examined, and the autoradiographic distribution of this ligand in brain sections was compared with the immunohistochemical localization of adenosine deaminase (ADA). It was found that [³H]NBI labels sites for which adenosine has far higher affinity than do other nucleosides, that these sites are heterogeneously distributed and that there is an exact correspondence between areas containing [³H]NBI sites and ADA-immunoreactive neurons. Our results indicate that [³H]NBI and ADA are potential markers for revealing anatomical sites at which actions of adenosine may be expressed.

Original language	English (US)
Pages (from-to)	47-53
Number of pages	7
Journal	Neuroscience Letters
Volume	55
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(85)90310-6
State	Published - Mar 22 1985
Externally published	Yes

Keywords

[H]nitrobenzylthioinosine
adenosine
autoradiography
deaminase
immunohistochemistry

ASJC Scopus subject areas

General Neuroscience

Access to Document

10.1016/0304-3940(85)90310-6

Cite this

@article{5f98a88a92a943478e20f7d552dea364,

title = "Adenosine uptake sites in rat brain: Identification using [3H]nitrobenzylthioinosine and co-localization with adenosine deaminase",

abstract = "The binding characteristics of [3H]nitrobenzylthioinosine ([3H]NBI) to rat brain membrane preparations was examined, and the autoradiographic distribution of this ligand in brain sections was compared with the immunohistochemical localization of adenosine deaminase (ADA). It was found that [3H]NBI labels sites for which adenosine has far higher affinity than do other nucleosides, that these sites are heterogeneously distributed and that there is an exact correspondence between areas containing [3H]NBI sites and ADA-immunoreactive neurons. Our results indicate that [3H]NBI and ADA are potential markers for revealing anatomical sites at which actions of adenosine may be expressed.",

keywords = "[H]nitrobenzylthioinosine, adenosine, autoradiography, deaminase, immunohistochemistry",

author = "Nagy, {J. I.} and Geiger, {J. D.} and Daddona, {P. E.}",

note = "Funding Information: We thank Mark Whittaker for technical assistance, and Lyn Poison and Carroll Taylor for typing the manuscript. This work was supported by grants from the Health Sciences Centre Research Foundation, the Manitoba Mental Health Research Foundations and the University of Manitoba Faculty Fund to J.I.N. and from the National Cancer Institute (Grant CA26284) to P.E.D.J.1.N. and J.D.G. are Scholars of the Canadian MRC and Manitoba Health Research Council, respectively.",

year = "1985",

month = mar,

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doi = "10.1016/0304-3940(85)90310-6",

language = "English (US)",

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pages = "47--53",

journal = "Neuroscience Letters",

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T1 - Adenosine uptake sites in rat brain

T2 - Identification using [3H]nitrobenzylthioinosine and co-localization with adenosine deaminase

AU - Nagy, J. I.

AU - Geiger, J. D.

AU - Daddona, P. E.

N1 - Funding Information: We thank Mark Whittaker for technical assistance, and Lyn Poison and Carroll Taylor for typing the manuscript. This work was supported by grants from the Health Sciences Centre Research Foundation, the Manitoba Mental Health Research Foundations and the University of Manitoba Faculty Fund to J.I.N. and from the National Cancer Institute (Grant CA26284) to P.E.D.J.1.N. and J.D.G. are Scholars of the Canadian MRC and Manitoba Health Research Council, respectively.

PY - 1985/3/22

Y1 - 1985/3/22

N2 - The binding characteristics of [3H]nitrobenzylthioinosine ([3H]NBI) to rat brain membrane preparations was examined, and the autoradiographic distribution of this ligand in brain sections was compared with the immunohistochemical localization of adenosine deaminase (ADA). It was found that [3H]NBI labels sites for which adenosine has far higher affinity than do other nucleosides, that these sites are heterogeneously distributed and that there is an exact correspondence between areas containing [3H]NBI sites and ADA-immunoreactive neurons. Our results indicate that [3H]NBI and ADA are potential markers for revealing anatomical sites at which actions of adenosine may be expressed.

AB - The binding characteristics of [3H]nitrobenzylthioinosine ([3H]NBI) to rat brain membrane preparations was examined, and the autoradiographic distribution of this ligand in brain sections was compared with the immunohistochemical localization of adenosine deaminase (ADA). It was found that [3H]NBI labels sites for which adenosine has far higher affinity than do other nucleosides, that these sites are heterogeneously distributed and that there is an exact correspondence between areas containing [3H]NBI sites and ADA-immunoreactive neurons. Our results indicate that [3H]NBI and ADA are potential markers for revealing anatomical sites at which actions of adenosine may be expressed.

KW - [H]nitrobenzylthioinosine

KW - adenosine

KW - autoradiography

KW - deaminase

KW - immunohistochemistry

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