Abstract
The binding characteristics of [3H]nitrobenzylthioinosine ([3H]NBI) to rat brain membrane preparations was examined, and the autoradiographic distribution of this ligand in brain sections was compared with the immunohistochemical localization of adenosine deaminase (ADA). It was found that [3H]NBI labels sites for which adenosine has far higher affinity than do other nucleosides, that these sites are heterogeneously distributed and that there is an exact correspondence between areas containing [3H]NBI sites and ADA-immunoreactive neurons. Our results indicate that [3H]NBI and ADA are potential markers for revealing anatomical sites at which actions of adenosine may be expressed.
Original language | English (US) |
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Pages (from-to) | 47-53 |
Number of pages | 7 |
Journal | Neuroscience Letters |
Volume | 55 |
Issue number | 1 |
DOIs | |
State | Published - Mar 22 1985 |
Externally published | Yes |
Keywords
- [H]nitrobenzylthioinosine
- adenosine
- autoradiography
- deaminase
- immunohistochemistry
ASJC Scopus subject areas
- Neuroscience(all)