Adenosine uptake sites in rat brain: Identification using [3H]nitrobenzylthioinosine and co-localization with adenosine deaminase

J. I. Nagy, J. D. Geiger, P. E. Daddona

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

The binding characteristics of [3H]nitrobenzylthioinosine ([3H]NBI) to rat brain membrane preparations was examined, and the autoradiographic distribution of this ligand in brain sections was compared with the immunohistochemical localization of adenosine deaminase (ADA). It was found that [3H]NBI labels sites for which adenosine has far higher affinity than do other nucleosides, that these sites are heterogeneously distributed and that there is an exact correspondence between areas containing [3H]NBI sites and ADA-immunoreactive neurons. Our results indicate that [3H]NBI and ADA are potential markers for revealing anatomical sites at which actions of adenosine may be expressed.

Original languageEnglish (US)
Pages (from-to)47-53
Number of pages7
JournalNeuroscience Letters
Volume55
Issue number1
DOIs
StatePublished - Mar 22 1985

Keywords

  • [H]nitrobenzylthioinosine
  • adenosine
  • autoradiography
  • deaminase
  • immunohistochemistry

ASJC Scopus subject areas

  • Neuroscience(all)

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