Adherence of platelets to in situ albumin-binding surfaces under flow conditions: Role of surface-adsorbed albumin

Sanjukta Guha Thakurta, Robert Miller, Anuradha Subramanian

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Surfaces that preferentially bind human serum albumin (HSA) were generated by grafting albumin-binding linear peptide (LP1) onto silicon surfaces. The research aim was to evaluate the adsorption pattern of proteins and the adhesion of platelets from platelet-poor plasma and platelet-rich plasma, respectively, by albumin-binding surfaces under physiological shear rate (96 and 319 s -1) conditions. Bound proteins were quantified using enzyme-linked immunosorbent assays (ELISAs) and two-dimensional gel electrophoresis. A ratio of ∼1000:100:1 of adsorbed HSA, human immunoglobulin (HIgG) and human fibrinogen (HFib) was noted, respectively, on LP1-functionalized surfaces, and a ratio of ∼5:2:1 of the same was noted on control surfaces, as confirmed by ELISAs. The surface-adsorbed von Willebrand factor was undetectable by sensitive ELISAs. The amount of adhered platelets correlated with the ratio of adsorbed HSA/HFib. Platelet morphology was more rounded on LP1-functionalized surfaces when compared to control surfaces. The platelet adhesion response on albumin-binding surfaces can be explained by the reduction in the co-adsorption of other plasma proteins in a surface environment where there is an excess of albumin molecules, coupled with restrictions in the conformational transitions of other surface-adsorbed proteins into hemostatically active forms.

Original languageEnglish (US)
Article number045007
JournalBiomedical Materials (Bristol)
Volume7
Issue number4
DOIs
StatePublished - Aug 2012

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

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