Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress

Marisa Zallocchi, Laura Matković, María C. Damasco

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

This work studied the effect of stresses produced by simulated gavage or gavage with 200 mmol/L HCl two hours before adrenal extraction, on the activities of the 11β-hydroxysteroid dehydrogenase 1 and 11β-hydroxysteroid dehydrogenase 2 isoforms present in the rat adrenal gland. These activities were determined on immediately prepared adrenal microsomes following incubations with 3H-corticosterone and NAD + or NADP+. 11-dehydrocorticosterone was measured as an end-product by TLC, and controls were adrenal microsomes from rats kept under basal (unstressed) conditions. 11β-hydroxysteroid dehydrogenase 1 activity, but not 11β-hydroxysteroid dehydrogenase 2 activity, was increased under both stress-conditions. Homeostatically, the stimulation of 11β- hydroxysteroid dehydrogenase 1 activity would increase the supply of glucocorticoids. These, in turn, would activate the enzyme phenylethanolamine N-methyl transferase, thereby improving the synthesis of epinephrine as part of the stress-response.

Original languageEnglish (US)
Pages (from-to)422-425
Number of pages4
JournalCanadian journal of physiology and pharmacology
Volume82
Issue number6
DOIs
StatePublished - Jun 2004

Keywords

  • Acidosis
  • Adrenal
  • HSD
  • Stress

ASJC Scopus subject areas

  • Physiology
  • Pharmacology
  • Physiology (medical)

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