Adsorption of human serum albumin in porous silicon gradients monitored by spatially-resolved spectroscopic ellipsometry

L. M. Karlsson, M. Schubert, N. Ashkenov, H. Arwin

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Porous silicon layers with a one-dimensional lateral gradient in pore size are prepared by electrochemical etching and characterized by spectroscopic ellipsometry in the visible to near-infrared region. The ellipsometer is equipped with a micro-spot option giving a lateral resolution of approximately 100 μm. By matching multiple-layer-model calculations to the laterally-resolved variable angle-of-incidence spectroscopic ellipsometry data, the thickness variation along the gradient as well as the in-depth porosity profile is mapped. Upon exposure to a protein solution, protein adsorption occurs on top of the porous silicon layer. At the high-porosity region of the gradient also penetration of protein molecules into the porous layer takes place. Ellipsometry data is recorded after protein exposure and variations of protein adsorption along the porous silicon gradient is modeled as well as the in-depth profile of protein penetration.

Original languageEnglish (US)
Pages (from-to)3293-3297
Number of pages5
JournalPhysica Status Solidi C: Conferences
Volume2
Issue number9
DOIs
StatePublished - 2005

ASJC Scopus subject areas

  • Condensed Matter Physics

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