Advanced microfluidic devices for cell electroporation and manipulation

Zaizai Dong, Tongren Yang, Han Wu, Justin Brooks, Ruiguo Yang, Yuanyu Huang, Lingqian Chang

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Microfluidic devices have shown capabilities for precise cell manipulation and analysis for decades. In recent years, the improvement of microfabrication technology and the introduction of new materials have boosted innovation on single-cell electroporation on microfluidic devices. This has opened the door for gene transfection and drug delivery with high efficiency and cellular safety in the exploration of cell biology and life science. In this chapter, we introduce the basic mechanism and state of the art of electroporation techniques based on microfluidic devices. We first discuss three types of electroporation systems for high-throughput cell transfection, including bulk electroporation, tubular microfluidic electroporation, and a localized electroporation device. Next, we shed light on cell manipulation techniques integrated on microfluidic electroporation devices to achieve higher efficiency and safer electroporation. Several methods for cell manipulation and their applications in electroporation for biomedicine are introduced.

Original languageEnglish (US)
Title of host publicationMicro and Nano Systems for Biophysical Studies of Cells and Small Organisms
PublisherElsevier
Pages105-123
Number of pages19
ISBN (Electronic)9780128239902
DOIs
StatePublished - Jan 1 2021

Keywords

  • Cell manipulation
  • Dielectrophoresis
  • Electroporation
  • Hydrodynamics
  • Lab-on-a-chip
  • Magnetic tweezers
  • Microfluidic device
  • Nanoelectroporation
  • Optical tweezers

ASJC Scopus subject areas

  • General Economics, Econometrics and Finance
  • General Business, Management and Accounting

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