Affinity chromatography: A historical perspective

David S. Hage, Ryan Matsuda

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Affinity chromatography is one of the most selective and versatile forms of liquid chromatography for the separation or analysis of chemicals in complex mixtures. This method makes use of a biologically related agent as the stationary phase, which provides an affinity column with the ability to bind selectively and reversibly to a given target in a sample. This review examines the early work in this method and various developments that have lead to the current status of this technique. The general principles of affinity chromatography are briefly described as part of this discussion. Past and recent efforts in the generation of new binding agents, supports, and immobilization methods for this method are considered. Various applications of affinity chromatography are also summarized, as well as the influence this field has played in the creation of other affinity-based separation or analysis methods.

Original languageEnglish (US)
JournalMethods in Molecular Biology
Volume1286
DOIs
StatePublished - 2015

Keywords

  • Affinity chromatography
  • Affinity monolith chromatography
  • Bioaffinity chromatography
  • Biomimetic affinity chromatography
  • Dye-ligand affinity chromatography
  • High-performance affinity chromatography
  • History
  • Immobilized metal-ion affinity chromatography
  • Immunoaffinity chromatography

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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