Affinity chromatography: A review of trends and developments over the past 50 years

Elliott L. Rodriguez, Saumen Poddar, Sazia Iftekhar, Kyungah Suh, Ashley G. Woolfork, Susan Ovbude, Allegra Pekarek, Morgan Walters, Shae Lott, David S. Hage

Research output: Contribution to journalReview articlepeer-review

6 Scopus citations


The field of affinity chromatography, which employs a biologically-related agent as the stationary phase, has seen significant growth since the modern era of this method began in 1968. This review examines the major developments and trends that have occurred in this technique over the past five decades. The basic principles and history of this area are first discussed. This is followed by an overview of the various supports, immobilization strategies, and types of binding agents that have been used in this field. The general types of applications and fields of use that have appeared for affinity chromatography are also considered. A survey of the literature is used to identify major trends in these topics and important areas of use for affinity chromatography in the separation, analysis, or characterization of chemicals and biochemicals.

Original languageEnglish (US)
Article number122332
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
StatePublished - Nov 10 2020


  • Affinity chromatography
  • Affinity ligands
  • Affinity supports
  • Applications
  • Immobilization methods

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology


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