TY - JOUR
T1 - Affinity monolith chromatography
T2 - A review of general principles and applications
AU - Li, Zhao
AU - Rodriguez, Elliott
AU - Azaria, Shiden
AU - Pekarek, Allegra
AU - Hage, David S.
N1 - Publisher Copyright:
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2017/11
Y1 - 2017/11
N2 - Affinity monolith chromatography, or AMC, is a liquid chromatographic method in which the support is a monolith and the stationary phase is a biological-binding agent or related mimic. AMC has become popular for the isolation of biochemicals, for the measurement of various analytes, and for studying biological interactions. This review will examine the principles and applications of AMC. The materials that have been used to prepare AMC columns will be discussed, which have included various organic polymers, silica, agarose, and cryogels. Immobilization schemes that have been used in AMC will also be considered. Various binding agents and applications that have been reported for AMC will then be described. These applications will include the use of AMC for bioaffinity chromatography, immunoaffinity chromatography, dye-ligand affinity chromatography, and immobilized metal-ion affinity chromatography. The use of AMC with chiral stationary phases and as a tool to characterize biological interactions will also be examined.
AB - Affinity monolith chromatography, or AMC, is a liquid chromatographic method in which the support is a monolith and the stationary phase is a biological-binding agent or related mimic. AMC has become popular for the isolation of biochemicals, for the measurement of various analytes, and for studying biological interactions. This review will examine the principles and applications of AMC. The materials that have been used to prepare AMC columns will be discussed, which have included various organic polymers, silica, agarose, and cryogels. Immobilization schemes that have been used in AMC will also be considered. Various binding agents and applications that have been reported for AMC will then be described. These applications will include the use of AMC for bioaffinity chromatography, immunoaffinity chromatography, dye-ligand affinity chromatography, and immobilized metal-ion affinity chromatography. The use of AMC with chiral stationary phases and as a tool to characterize biological interactions will also be examined.
KW - Affinity monolith chromatography
KW - Bioaffinity chromatography
KW - Chiral separations
KW - Dye-ligand affinity chromatography
KW - Immunoaffinity chromatography
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U2 - 10.1002/elps.201700101
DO - 10.1002/elps.201700101
M3 - Review article
C2 - 28474739
AN - SCOPUS:85019870498
SN - 0173-0835
VL - 38
SP - 2837
EP - 2850
JO - ELECTROPHORESIS
JF - ELECTROPHORESIS
IS - 22-23
ER -