Affinity monolith chromatography: A review of principles and recent analytical applications

Erika L. Pfaunmiller, Marie Laura Paulemond, Courtney M. Dupper, David S. Hage

Research output: Contribution to journalReview articlepeer-review

119 Scopus citations


Affinity monolith chromatography (AMC) is a type of liquid chromatography that uses a monolithic support and a biologically related binding agent as a stationary phase. AMC is a powerful method for the selective separation, analysis, or study of specific target compounds in a sample. This review discusses the basic principles of AMC and recent developments and applications of this method, with particular emphasis being given to work that has appeared in the last 5 years. Various materials that have been used to prepare columns for AMC are examined, including organic monoliths, silica monoliths, agarose monoliths, and cryogels. These supports have been used in AMC for formats that have ranged from traditional columns to disks, microcolumns, and capillaries. Many binding agents have also been employed in AMC, such as antibodies, enzymes, proteins, lectins, immobilized metal ions, and dyes. Some applications that have been reported with these binding agents in AMC are bioaffinity chromatography, immunoaffinity chromatography or immunoextraction, immobilized-metal-ion affinity chromatography, dye-ligand affinity chromatography, chiral separations, and biointeraction studies. Examples are presented from fields that include analytical chemistry, pharmaceutical analysis, clinical testing, and biotechnology. Current trends and possible directions in AMC are also discussed.

Original languageEnglish (US)
Pages (from-to)2133-2145
Number of pages13
JournalAnalytical and Bioanalytical Chemistry
Issue number7
StatePublished - Mar 2013
Externally publishedYes


  • Affinity chromatography
  • Affinity monolith chromatography
  • Bioaffinity chromatography
  • Biointeraction chromatography
  • Dye-ligand affinity chromatography
  • Immobilized-metal-ion affinity chromatography
  • Immunoaffinity chromatography
  • Monolithic supports

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry


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