TY - JOUR
T1 - AFLP genotyping and fingerprinting
AU - Mueller, Ulrich G.
AU - Wolfenbarger, L. La Reesa
N1 - Funding Information:
We thank Rachelle Adams, Eduardo Eizirik, Dina Fonseca, John McCarty, David Queller, Gerald Wilkinson and Tim Wright for comments. We are grateful to the NSF (DEB-9707209) and the NSF/Sloan Postdoctoral Fellowship Program in Molecular Evolution for support during the writing of this review.
PY - 1999/10/1
Y1 - 1999/10/1
N2 - Amplified fragment length polymorphisms (AFLPs) are polymerase chain reaction (PCR)-based markers for the rapid screening of genetic diversity. AFLP methods rapidly generate hundreds of highly replicable markers from DNA of any organism; thus, they allow high-resolution genotyping of fingerprinting quality. The time and cost efficiency, replicability and resolution of AFLPs are superior or equal to those of other markers [allozymes, random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP), microsatellites], except that AFLP methods primarily generate dominant rather than co- dominant markers. Because of their high replicability and ease of use, AFLP markers have emerged as a major new type of genetic marker with broad application in systematics, pathotyping, population genetics, DNA fingerprinting and quantitative trait loci (QTL) mapping.
AB - Amplified fragment length polymorphisms (AFLPs) are polymerase chain reaction (PCR)-based markers for the rapid screening of genetic diversity. AFLP methods rapidly generate hundreds of highly replicable markers from DNA of any organism; thus, they allow high-resolution genotyping of fingerprinting quality. The time and cost efficiency, replicability and resolution of AFLPs are superior or equal to those of other markers [allozymes, random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP), microsatellites], except that AFLP methods primarily generate dominant rather than co- dominant markers. Because of their high replicability and ease of use, AFLP markers have emerged as a major new type of genetic marker with broad application in systematics, pathotyping, population genetics, DNA fingerprinting and quantitative trait loci (QTL) mapping.
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U2 - 10.1016/S0169-5347(99)01659-6
DO - 10.1016/S0169-5347(99)01659-6
M3 - Review article
AN - SCOPUS:0033214958
SN - 0169-5347
VL - 14
SP - 389
EP - 394
JO - Trends in Ecology and Evolution
JF - Trends in Ecology and Evolution
IS - 10
ER -