Airborne agricultural particulate matter induces inflammatory cytokine secretion by respiratory epithelial cells: Mechanisms of regulation by eicosanoid lipid signal mediators

Smitha Malireddy, Courtney Lawson, Emily Steinhour, Judy Hart, Sainath R. Kotha, Rishi B. Patel, Lingying Zhao, John R. Wilkins, Clay B. Marsh, Ulysses J. Magalang, Debra Romberger, Mark D. Wewers, Narasimham L. Parinandi

Research output: Contribution to journalArticlepeer-review

Abstract

The purpose of this study was to elucidate the mechanism of the airborne poultry dust (particulate matter, PM)-induced respiratory tract inflammation, a common symptom in agricultural respiratory diseases. The study was based on the hypothesis that poultry PM would induce the release of inflammatory cytokine interleukin-8 (IL-8) by respiratory epithelial cells under the upstream regulation by cytosolic phospholipase A2 (cPLA2) activation and subsequent formation of cyclooxygenase (COX)- and lipoxygenase (LOX)-catalyzed arachidonic acid (AA) metabolites (eicosanoids). Human lung epithelial cells (A549) in culture were treated with the poultry PM (0.1-1.0 mg) for different lengths of time, following which PLA2 activity, release of eicosanoids and secretion of IL-8 in cells were determined. Poultry PM (1.0 mg/ml) caused a significant activation of PLA2 in a time-dependent manner (15-60 min), which was significantly attenuated by the calcium-chelating agents, cPLA2-specific inhibitor (AACOCF3) and antioxidant (vitamin C) in A549 cells. Poultry PM also significantly induced the release of COX- and LOX-catalyzed eicosanoids (prostaglandins, thromboxane A2 and leukotrienes B4 and C4) and upstream activation of AA LOX in the cells. Poultry PM also significantly induced release of IL-8 by the cells in a dose- and time-dependent manner, which was significantly attenuated by the calcium chelating agents, antioxidants and COX- and LOX-specific inhibitors. The current study for the first time revealed that the poultry PM-induced IL-8 release from the respiratory epithelial cells was regulated upstream by reactive oxygen species, cPLA2-, COX- and LOX-derived eicosanoid lipid signal mediators.

Original languageEnglish (US)
Pages (from-to)387-401
Number of pages15
JournalIndian Journal of Geo-Marine Sciences
Volume50
Issue number5
StatePublished - 2013

Keywords

  • Interleukin-8
  • Leukotrienes
  • Lung epithelial cell
  • Occupational farm respiratory diseases
  • Organic poultry dust
  • Phospholipase A
  • Prostaglandins

ASJC Scopus subject areas

  • Oceanography

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