Allelic exchange

Mckenzie K. Lehman, Jeffrey L. Bose, Kenneth W Bayles

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

Methods used to understand the function of a gene/protein are one of the hallmarks of modern molecular genetics. The ability to genetically manipulate bacteria has become a fundamental tool in studying these organisms and while basic cloning has become a routine task in molecular biology laboratories, generating directed mutations can be a daunting task. This chapter describes the method of allelic exchange in Staphylococcus aureus using temperature-sensitive plasmids that have successfully produced a variety of chromosomal mutations, including in-frame deletions, insertion of antibiotic-resistance cassettes, and even single-nucleotide point mutations.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages89-96
Number of pages8
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1373
ISSN (Print)1064-3745

Keywords

  • Allelic exchange
  • Cloning
  • Homologous recombination
  • Mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Lehman, M. K., Bose, J. L., & Bayles, K. W. (2016). Allelic exchange. In Methods in Molecular Biology (pp. 89-96). (Methods in Molecular Biology; Vol. 1373). Humana Press Inc.. https://doi.org/10.1007/7651_2014_187