Abstract
Methods used to understand the function of a gene/protein are one of the hallmarks of modern molecular genetics. The ability to genetically manipulate bacteria has become a fundamental tool in studying these organisms and while basic cloning has become a routine task in molecular biology laboratories, generating directed mutations can be a daunting task. This chapter describes the method of allelic exchange in Staphylococcus aureus using temperature-sensitive plasmids that have successfully produced a variety of chromosomal mutations, including in-frame deletions, insertion of antibiotic-resistance cassettes, and even single-nucleotide point mutations.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 89-96 |
| Number of pages | 8 |
| DOIs | |
| State | Published - 2016 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1373 |
| ISSN (Print) | 1064-3745 |
Keywords
- Allelic exchange
- Cloning
- Homologous recombination
- Mutation
ASJC Scopus subject areas
- Molecular Biology
- Genetics
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Cite this
Lehman, M. K., Bose, J. L., & Bayles, K. W. (2016). Allelic exchange. In Methods in Molecular Biology (pp. 89-96). (Methods in Molecular Biology; Vol. 1373). Humana Press Inc.. https://doi.org/10.1007/7651_2014_187