@inbook{b5e036d70ddf4190b588d4a65e065eaf,
title = "Allelic exchange",
abstract = "Methods used to understand the function of a gene/protein are one of the hallmarks of modern molecular genetics. The ability to genetically manipulate bacteria has become a fundamental tool in studying these organisms and while basic cloning has become a routine task in molecular biology laboratories, generating directed mutations can be a daunting task. This chapter describes the method of allelic exchange in Staphylococcus aureus using temperature-sensitive plasmids that have successfully produced a variety of chromosomal mutations, including in-frame deletions, insertion of antibiotic-resistance cassettes, and even single-nucleotide point mutations.",
keywords = "Allelic exchange, Cloning, Homologous recombination, Mutation",
author = "Lehman, {Mckenzie K.} and Bose, {Jeffrey L.} and Bayles, {Kenneth W}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2014.",
year = "2016",
doi = "10.1007/7651_2014_187",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "89--96",
booktitle = "Methods in Molecular Biology",
}