Allelic variants of ovine prion protein gene (PRNP) in Oklahoma sheep

U. DeSilva; X. Guo; D. M. Kupfer; S. C. Fernando; A. T.V. Pillai; F. Z. Najar; S. So; G. Q. Fitch; B. A. Roe

doi:10.1159/000075731

Allelic variants of ovine prion protein gene (PRNP) in Oklahoma sheep

U. DeSilva, X. Guo, D. M. Kupfer, S. C. Fernando, A. T.V. Pillai, F. Z. Najar, S. So, G. Q. Fitch, B. A. Roe

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

1,144 sheep belonging to 21 breeds and known crosses were sequence analyzed for polymorphisms in the ovine PRNP gene. Genotype and allele frequencies of polymorphisms in PRNP known to confer resistance to scrapie, a fatal neurodegenerative disease of sheep, are reported. Known polymorphisms at codons 136 (A/V), 154 (H/R) and 171 (Q/R/H/K) were identified. The frequency of the 171R allele known to confer resistance to type C scrapie was 53.8% and the frequency of the 136A allele known to influence the resistance to type A scrapie was 96.01%. In addition, we report the identification of five new polymorphisms at codons 143 (H/R), 167 (R/S), 180 (H/Y), 195 (T/S) and 196 (T/S). We also report the identification of a novel allele (S/R) at codon 138.

Original language	English (US)
Pages (from-to)	89-94
Number of pages	6
Journal	Cytogenetic and Genome Research
Volume	102
Issue number	1-4
DOIs	https://doi.org/10.1159/000075731
State	Published - 2003
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Genetics
Genetics(clinical)

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@article{2494da23464a4f0da6ae59bba1c91052,

title = "Allelic variants of ovine prion protein gene (PRNP) in Oklahoma sheep",

abstract = "1,144 sheep belonging to 21 breeds and known crosses were sequence analyzed for polymorphisms in the ovine PRNP gene. Genotype and allele frequencies of polymorphisms in PRNP known to confer resistance to scrapie, a fatal neurodegenerative disease of sheep, are reported. Known polymorphisms at codons 136 (A/V), 154 (H/R) and 171 (Q/R/H/K) were identified. The frequency of the 171R allele known to confer resistance to type C scrapie was 53.8% and the frequency of the 136A allele known to influence the resistance to type A scrapie was 96.01%. In addition, we report the identification of five new polymorphisms at codons 143 (H/R), 167 (R/S), 180 (H/Y), 195 (T/S) and 196 (T/S). We also report the identification of a novel allele (S/R) at codon 138.",

author = "U. DeSilva and X. Guo and Kupfer, {D. M.} and Fernando, {S. C.} and Pillai, {A. T.V.} and Najar, {F. Z.} and S. So and Fitch, {G. Q.} and Roe, {B. A.}",

year = "2003",

doi = "10.1159/000075731",

language = "English (US)",

volume = "102",

pages = "89--94",

journal = "Cytogenetic and Genome Research",

issn = "1424-8581",

publisher = "S. Karger AG",

number = "1-4",

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TY - JOUR

T1 - Allelic variants of ovine prion protein gene (PRNP) in Oklahoma sheep

AU - DeSilva, U.

AU - Guo, X.

AU - Kupfer, D. M.

AU - Fernando, S. C.

AU - Pillai, A. T.V.

AU - Najar, F. Z.

AU - So, S.

AU - Fitch, G. Q.

AU - Roe, B. A.

PY - 2003

Y1 - 2003

N2 - 1,144 sheep belonging to 21 breeds and known crosses were sequence analyzed for polymorphisms in the ovine PRNP gene. Genotype and allele frequencies of polymorphisms in PRNP known to confer resistance to scrapie, a fatal neurodegenerative disease of sheep, are reported. Known polymorphisms at codons 136 (A/V), 154 (H/R) and 171 (Q/R/H/K) were identified. The frequency of the 171R allele known to confer resistance to type C scrapie was 53.8% and the frequency of the 136A allele known to influence the resistance to type A scrapie was 96.01%. In addition, we report the identification of five new polymorphisms at codons 143 (H/R), 167 (R/S), 180 (H/Y), 195 (T/S) and 196 (T/S). We also report the identification of a novel allele (S/R) at codon 138.

AB - 1,144 sheep belonging to 21 breeds and known crosses were sequence analyzed for polymorphisms in the ovine PRNP gene. Genotype and allele frequencies of polymorphisms in PRNP known to confer resistance to scrapie, a fatal neurodegenerative disease of sheep, are reported. Known polymorphisms at codons 136 (A/V), 154 (H/R) and 171 (Q/R/H/K) were identified. The frequency of the 171R allele known to confer resistance to type C scrapie was 53.8% and the frequency of the 136A allele known to influence the resistance to type A scrapie was 96.01%. In addition, we report the identification of five new polymorphisms at codons 143 (H/R), 167 (R/S), 180 (H/Y), 195 (T/S) and 196 (T/S). We also report the identification of a novel allele (S/R) at codon 138.

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JO - Cytogenetic and Genome Research

JF - Cytogenetic and Genome Research

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ER -

Allelic variants of ovine prion protein gene (PRNP) in Oklahoma sheep

Abstract

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