Allergenicity of various peanut products as determined by RAST inhibition

Julie A. Nordlee; Steve L. Taylor; R. T. Jones; John W. Yunginger

doi:10.1016/0091-6749(81)90136-6

Allergenicity of various peanut products as determined by RAST inhibition

Julie A. Nordlee, Steve L. Taylor, R. T. Jones, John W. Yunginger

Research output: Contribution to journal › Article › peer-review

104 Scopus citations

Abstract

Extracts of 19 different peanut products and peanut oil were tested for their allergenicity by the radioallergosorbent test inhibition assay using a crude peanut extract from raw peanuts as the standard for comparison. Seventeen of the extracts were able to competitively inhibit the binding of serum IgE from peanut-sensitive patients with the solid-phase raw peanut extract. Peanut oil and the extract from hydrolyzed peanut protein did not inhibit binding, which suggests that these products are not allergenic. The peanut hull flour extract showed a slight ability to inhibit binding, suggesting that this product contains minor amounts of the peanut allergen.

Original language	English (US)
Pages (from-to)	376-382
Number of pages	7
Journal	The Journal of Allergy and Clinical Immunology
Volume	68
Issue number	5
DOIs	https://doi.org/10.1016/0091-6749(81)90136-6
State	Published - Nov 1981
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0091-6749(81)90136-6

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@article{7cc63b45743740149dabad4c4e559d1e,

title = "Allergenicity of various peanut products as determined by RAST inhibition",

abstract = "Extracts of 19 different peanut products and peanut oil were tested for their allergenicity by the radioallergosorbent test inhibition assay using a crude peanut extract from raw peanuts as the standard for comparison. Seventeen of the extracts were able to competitively inhibit the binding of serum IgE from peanut-sensitive patients with the solid-phase raw peanut extract. Peanut oil and the extract from hydrolyzed peanut protein did not inhibit binding, which suggests that these products are not allergenic. The peanut hull flour extract showed a slight ability to inhibit binding, suggesting that this product contains minor amounts of the peanut allergen.",

author = "Nordlee, {Julie A.} and Taylor, {Steve L.} and Jones, {R. T.} and Yunginger, {John W.}",

note = "Funding Information: From the Food Research Institute, Department of Food Mi-crobiology and Toxicology, and the Department of Food Sci-ence, University of Wisconsin; and Department of Pediatrics and Allergic Diseases Research Laboratory, Mayo Graduate School of Medicine, Mayo Clinic and Foundation. Supported in part by contributions from the food industry and by the College of Agricultural and Life Sciences, University of Wisconsin. Received for publication April 27, 198 1. Accepted for publication July 3 1, 198 1. Reprint requests to: Steve L. Taylor, Ph.D., Food Research Insti-tute, University of Wisconsin, Madison, WI 53706.",

year = "1981",

month = nov,

doi = "10.1016/0091-6749(81)90136-6",

language = "English (US)",

volume = "68",

pages = "376--382",

journal = "The Journal of Allergy and Clinical Immunology",

issn = "0091-6749",

publisher = "Elsevier Inc.",

number = "5",

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TY - JOUR

T1 - Allergenicity of various peanut products as determined by RAST inhibition

AU - Nordlee, Julie A.

AU - Taylor, Steve L.

AU - Jones, R. T.

AU - Yunginger, John W.

N1 - Funding Information: From the Food Research Institute, Department of Food Mi-crobiology and Toxicology, and the Department of Food Sci-ence, University of Wisconsin; and Department of Pediatrics and Allergic Diseases Research Laboratory, Mayo Graduate School of Medicine, Mayo Clinic and Foundation. Supported in part by contributions from the food industry and by the College of Agricultural and Life Sciences, University of Wisconsin. Received for publication April 27, 198 1. Accepted for publication July 3 1, 198 1. Reprint requests to: Steve L. Taylor, Ph.D., Food Research Insti-tute, University of Wisconsin, Madison, WI 53706.

PY - 1981/11

Y1 - 1981/11

N2 - Extracts of 19 different peanut products and peanut oil were tested for their allergenicity by the radioallergosorbent test inhibition assay using a crude peanut extract from raw peanuts as the standard for comparison. Seventeen of the extracts were able to competitively inhibit the binding of serum IgE from peanut-sensitive patients with the solid-phase raw peanut extract. Peanut oil and the extract from hydrolyzed peanut protein did not inhibit binding, which suggests that these products are not allergenic. The peanut hull flour extract showed a slight ability to inhibit binding, suggesting that this product contains minor amounts of the peanut allergen.

AB - Extracts of 19 different peanut products and peanut oil were tested for their allergenicity by the radioallergosorbent test inhibition assay using a crude peanut extract from raw peanuts as the standard for comparison. Seventeen of the extracts were able to competitively inhibit the binding of serum IgE from peanut-sensitive patients with the solid-phase raw peanut extract. Peanut oil and the extract from hydrolyzed peanut protein did not inhibit binding, which suggests that these products are not allergenic. The peanut hull flour extract showed a slight ability to inhibit binding, suggesting that this product contains minor amounts of the peanut allergen.

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JO - The Journal of Allergy and Clinical Immunology

JF - The Journal of Allergy and Clinical Immunology

IS - 5

ER -

Allergenicity of various peanut products as determined by RAST inhibition

Abstract

ASJC Scopus subject areas

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