TY - JOUR
T1 - Alpha interferon-induced antiretroviral activities
T2 - Restriction of viral nucleic acid synthesis and progeny virion production in human immunodeficiency virus type 1-infected monocytes
AU - Baca-Regen, Lisa
AU - Heinzinger, Nina
AU - Stevenson, Mario
AU - Gendelman, Howard E.
PY - 1994/11
Y1 - 1994/11
N2 - Alpha interferon (IFN-α) restricts multiple steps of the human immunodeficiency virus type 1 (HIV-1) life cycle. A well-described effect of IFN-α is in the modulation of viral nucleic acid synthesis. We demonstrate that IFN-α influences HIV-1 DNA synthesis principally by reducing the production of late products of reverse transcription. The magnitude of IFN- α-induced downregulation of HIV-1 DNA and/or progeny virion production was dependent on the IFN-α concentration, the duration of cytokine administration, the multiplicity of infection, the viral strain, and the cycles of viral infection. Interestingly, reductions in viral DNAs could not fully account for the observed IFN-α-induced abrogation of progeny virion production. These data, by our investigation of both single-cycle and spreading viral infections, support a predominant but not exclusive effect of IFN-α on viral DNA synthesis.
AB - Alpha interferon (IFN-α) restricts multiple steps of the human immunodeficiency virus type 1 (HIV-1) life cycle. A well-described effect of IFN-α is in the modulation of viral nucleic acid synthesis. We demonstrate that IFN-α influences HIV-1 DNA synthesis principally by reducing the production of late products of reverse transcription. The magnitude of IFN- α-induced downregulation of HIV-1 DNA and/or progeny virion production was dependent on the IFN-α concentration, the duration of cytokine administration, the multiplicity of infection, the viral strain, and the cycles of viral infection. Interestingly, reductions in viral DNAs could not fully account for the observed IFN-α-induced abrogation of progeny virion production. These data, by our investigation of both single-cycle and spreading viral infections, support a predominant but not exclusive effect of IFN-α on viral DNA synthesis.
UR - http://www.scopus.com/inward/record.url?scp=0028063447&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028063447&partnerID=8YFLogxK
U2 - 10.1128/jvi.68.11.7559-7565.1994
DO - 10.1128/jvi.68.11.7559-7565.1994
M3 - Comment/debate
C2 - 7933143
AN - SCOPUS:0028063447
SN - 0022-538X
VL - 68
SP - 7559
EP - 7565
JO - Journal of virology
JF - Journal of virology
IS - 11
ER -