Alterations in P-Glycoprotein Expression and Function Between Macrophage Subsets

Theodore J. Cory, Hui He, Lee C. Winchester, Santosh Kumar, Courtney V. Fletcher

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Purpose: Macrophages are an important cellular reservoir in HIV, and exist in two phenotypically dissimilar subsets, the pro-inflammatory M1 phenotype, and the anti-inflammatory M2 phenotype. The role of these two subsets is uncertain. We hypothesized that differences in drug efflux transporters exist between the subsets, which would result in altered intracellular drug concentrations between these cells. Methods: U937 monocytic cells were polarized to the M1 or M2 phenotype via treatment with interferon-gamma and LPS, or interleukins 4, 13, and LPS, respectively. PGP function was assessed with Hoechst 33342, and expression via western blotting. Intracellular lopinavir was assessed via LC-MS/MS. Data was confirmed with primary monocyte derived macrophages. Results: We observed significant differences in intracellular concentrations of lopinavir, a PGP substrate, with higher concentrations in M1 cells. PGP function and expression was higher in the M2 macrophages. These results were confirmed with primary monocyte derived macrophages. Conclusions: This data shows that there are previously unreported differences in P-glycoprotein expression between macrophage subsets, and suggests that there may be differences for other transporters. These differences can play a role in intracellular drug concentrations in these cells, and may allow for low-level HIV replication.

Original languageEnglish (US)
Pages (from-to)2713-2721
Number of pages9
JournalPharmaceutical Research
Volume33
Issue number11
DOIs
StatePublished - Nov 1 2016

Keywords

  • Hoechst 33342
  • P-glycoprotein
  • alternatively activated macrophage
  • classically activated macrophage

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry
  • Pharmacology (medical)

Fingerprint Dive into the research topics of 'Alterations in P-Glycoprotein Expression and Function Between Macrophage Subsets'. Together they form a unique fingerprint.

Cite this