TY - JOUR
T1 - Alterations in the nuclear proteome of HIV-1 infected T-cells
AU - DeBoer, Jason
AU - Jagadish, Teena
AU - Haverland, Nicole A.
AU - Madson, Christian J.
AU - Ciborowski, Pawel
AU - Belshan, Michael
N1 - Funding Information:
This work was supported by grants from the National Institutes of Health, USA ( AI080348 (M.B.) and DA030962 (P.C.)). Jason DeBoer is funded by the US Army Medical Service Corp Long Term Health Education Program .
Publisher Copyright:
© 2014 Elsevier Inc.
PY - 2014/11/1
Y1 - 2014/11/1
N2 - Virus infection of a cell involves the appropriation of host factors and the innate defensive response of the cell. The identification of proteins critical for virus replication may lead to the development of novel, cell-based inhibitors. In this study we mapped the changes in T-cell nuclei during human immunodeficiency virus type 1 (HIV-1) at 20. hpi. Using a stringent data threshold, a total of 13 and 38 unique proteins were identified in infected and uninfected cells, respectively, across all biological replicates. An additional 15 proteins were found to be differentially regulated between infected and control nuclei. STRING analysis identified four clusters of protein-protein interactions in the data set related to nuclear architecture, RNA regulation, cell division, and cell homeostasis. Immunoblot analysis confirmed the differential expression of several proteins in both C8166-45 and Jurkat E6-1 T-cells. These data provide a map of the response in host cell nuclei upon HIV-1 infection.
AB - Virus infection of a cell involves the appropriation of host factors and the innate defensive response of the cell. The identification of proteins critical for virus replication may lead to the development of novel, cell-based inhibitors. In this study we mapped the changes in T-cell nuclei during human immunodeficiency virus type 1 (HIV-1) at 20. hpi. Using a stringent data threshold, a total of 13 and 38 unique proteins were identified in infected and uninfected cells, respectively, across all biological replicates. An additional 15 proteins were found to be differentially regulated between infected and control nuclei. STRING analysis identified four clusters of protein-protein interactions in the data set related to nuclear architecture, RNA regulation, cell division, and cell homeostasis. Immunoblot analysis confirmed the differential expression of several proteins in both C8166-45 and Jurkat E6-1 T-cells. These data provide a map of the response in host cell nuclei upon HIV-1 infection.
KW - HIV-1
KW - Label-free MS/MS
KW - Nuclear isolation
KW - Proteomics
KW - Virus-host interactions
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U2 - 10.1016/j.virol.2014.08.029
DO - 10.1016/j.virol.2014.08.029
M3 - Article
C2 - 25240327
AN - SCOPUS:84907450611
SN - 0042-6822
VL - 468
SP - 409
EP - 420
JO - Virology
JF - Virology
ER -