Alterations in the nuclear proteome of HIV-1 infected T-cells

Jason DeBoer, Teena Jagadish, Nicole A. Haverland, Christian J. Madson, Pawel Ciborowski, Michael Belshan

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Virus infection of a cell involves the appropriation of host factors and the innate defensive response of the cell. The identification of proteins critical for virus replication may lead to the development of novel, cell-based inhibitors. In this study we mapped the changes in T-cell nuclei during human immunodeficiency virus type 1 (HIV-1) at 20. hpi. Using a stringent data threshold, a total of 13 and 38 unique proteins were identified in infected and uninfected cells, respectively, across all biological replicates. An additional 15 proteins were found to be differentially regulated between infected and control nuclei. STRING analysis identified four clusters of protein-protein interactions in the data set related to nuclear architecture, RNA regulation, cell division, and cell homeostasis. Immunoblot analysis confirmed the differential expression of several proteins in both C8166-45 and Jurkat E6-1 T-cells. These data provide a map of the response in host cell nuclei upon HIV-1 infection.

Original languageEnglish (US)
Pages (from-to)409-420
Number of pages12
JournalVirology
Volume468
DOIs
StatePublished - Nov 1 2014

Keywords

  • HIV-1
  • Label-free MS/MS
  • Nuclear isolation
  • Proteomics
  • Virus-host interactions

ASJC Scopus subject areas

  • Virology

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    DeBoer, J., Jagadish, T., Haverland, N. A., Madson, C. J., Ciborowski, P., & Belshan, M. (2014). Alterations in the nuclear proteome of HIV-1 infected T-cells. Virology, 468, 409-420. https://doi.org/10.1016/j.virol.2014.08.029