TY - JOUR
T1 - Altered expression of MUC2 and MUC5AC in response to Shigella infection, an in vivo study
AU - Radhakrishnan, Prakash
AU - Halagowder, Devaraj
AU - Devaraj, S. Niranjali
N1 - Funding Information:
This work was supported by the University Grants Commission (UGC), New Delhi, India.
PY - 2007/6
Y1 - 2007/6
N2 - Infection of mucosal epithelial cells by Shigella species leads to an intense and acute inflammatory bowel disease that is characterized by watery diarrhea and purulent discharge. Mucin production is a common defense mechanism to protect the underlying mucosa against pathogens. The molecular mechanism(s) underlying mucin induction is unknown in Shigellosis. In this study, we have evaluated the relationship between Shigella infection, the expression of MUC2 and MUC5AC and the participation of signaling molecules TNF-α, PKC and ERK1/2. Shigella infection up-regulated MUC2 and MUC5AC expression in 6-8 h, through activation of TNF-α, PKC and ERK1/2. These results confirm that, in response to Shigella infection, the normal expression pattern of MUC-2 and MUC-5AC is altered. This in vivo study brings new insights into the molecular pathogenesis of Shigellosis and new potential therapeutic targets for Shigellosis.
AB - Infection of mucosal epithelial cells by Shigella species leads to an intense and acute inflammatory bowel disease that is characterized by watery diarrhea and purulent discharge. Mucin production is a common defense mechanism to protect the underlying mucosa against pathogens. The molecular mechanism(s) underlying mucin induction is unknown in Shigellosis. In this study, we have evaluated the relationship between Shigella infection, the expression of MUC2 and MUC5AC and the participation of signaling molecules TNF-α, PKC and ERK1/2. Shigella infection up-regulated MUC2 and MUC5AC expression in 6-8 h, through activation of TNF-α, PKC and ERK1/2. These results confirm that, in response to Shigella infection, the normal expression pattern of MUC-2 and MUC-5AC is altered. This in vivo study brings new insights into the molecular pathogenesis of Shigellosis and new potential therapeutic targets for Shigellosis.
KW - Ileal loop ligation assay
KW - MUC2
KW - MUC5AC
KW - Shigella species
KW - Shigellosis
UR - http://www.scopus.com/inward/record.url?scp=34247219571&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34247219571&partnerID=8YFLogxK
U2 - 10.1016/j.bbagen.2007.01.019
DO - 10.1016/j.bbagen.2007.01.019
M3 - Article
C2 - 17395379
AN - SCOPUS:34247219571
SN - 0304-4165
VL - 1770
SP - 884
EP - 889
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
IS - 6
ER -