Altered expression of type I collagen, TGF-β1, and related genes in rat lung exposed to 85% O2

A. M. Moore, S. Buch, R. N.N. Han, B. A. Freeman, M. Post, A. K. Tanswell

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

The gene expressions of type I collagen and transforming growth factor- β1 (TGF-β1) were studied in lung tissue of rats exposed to air or 85% O2 for 14 days. Peak expression of type I collagen mRNA was observed by 14 days of 85% O2 exposure, at the same time as maximal immunoreactive type I collagen, which was most marked surrounding the major airways and vessels. TGF-β1 mRNA also significantly increased after 14, but not 4 or 6, days of 85% O2 exposure. TGF-β1 immunoreactivity was only detected on day 14 of 85% O2 exposure and was localized primarily to the pulmonary epithelium. As an increase in immunoreactive type I collagen was evident by day 6 of O2 exposure, the gene expressions of interstitial collagenase (MMP-1), stromelysin, and the tissue inhibitor of the metalloproteinases (TIMP) were also examined. Increased mRNA expressions of interstitial collagenase and TIMP preceded those of type I collagen and TGF-β1, occurring at 4-6 days of exposure to 85% O2, while there was no significant change in stromelysin mRNA. These findings are compatible with the initial O2-mediated increase in type I collagen deposition being a result of an altered proteinase/antiproteinase balance in the lung, and the subsequent more marked deposition being a response to increased TGF-β1 synthesis.

Original languageEnglish (US)
Pages (from-to)L78-L84
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume268
Issue number1 12-1
StatePublished - Jan 1 1995

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

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