rTS is a gene with convergent and overlapping transcription relative to thymidylate synthase (TS). Screening of a cDNA library has identified a second rTS RNA (rTSβ) which is related to a previously described rTS RNA (rTSα). rTSβ differs from rTSα by the insertion of an extra 116 nucleotides after base 128 of rTSα and an altered 3'-noncoding region such that rTSβ RNA is not complementary to TS mRNA. rTSα and rTSβ RNAs have open reading frames predicted to encode proteins of 361 and 416 amino acids, respectively. Antibody to rTSβ protein detects two proteins of the predicted molecular weight in the H630 colon tumor cell line. rTSβ is overexpressed 40-70-fold in a 5-fluorouracil-resistant H630 subline compared with 7-8-fold for TS and rTSα.
|Original language||English (US)|
|Number of pages||4|
|State||Published - Jul 15 1996|
ASJC Scopus subject areas
- Cancer Research